Method for Increasing Intracellular Heme Content of Escherichia coli
AssigneeJiangnan University
InventorsLei Tang, Mei Pan, Shuangxin Liu
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Abstract
The disclosure discloses a method for increasing the intracellular heme content of Escherichia coli and belongs to the field of metabolic engineering. In the disclosure, in E. coli, the gene mscS encoding a small conductance mechanically sensitive ion channel protein is knocked out, the gene aroG encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase is knocked out, or the gene hemA encoding glutamyl-tRNA reductase is overexpressed. The constructed recombinant strain is cultured in an LB culture medium, and the heme content can reach 47.6 μmol·L−1, which is significantly higher than that of a control strain. The recombinant strain has the value of wide application.
Claims8
Recombinant strain of Escherichia coli(E. Coli) comprising one or more of the following mutated genes, wherein the gene mscS encoding a small conductance mechanically sensitive ion channel protein is knocked out or silenced, or the gene aroG encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase is knocked out or silenced, or the gene hemA encoding glutamyl-tRNA reductase is overexpressed.
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