Controlled Release Vaccines and Methods of Treating Brucella Diseases and Disorders

A method for prophylaxis, immunization, amelioration of symptoms, or any combinations thereof against brucellosis in a human or animal subject comprising the steps of:

• identifying the human or animal subject in need of the prophylaxis, immunization, amelioration of symptoms, or any combinations thereof against brucellosis;
• and
• administering a therapeutically effective amount of a vaccine composition to the human or animal subject for the prophylaxis, amelioration of symptoms, or any combinations thereof against brucellosis, wherein the vaccine comprises:
• a Brucella strain comprising one or more attenuating gene knockouts and further comprising a diagnostic gene knockout;
• an encapsulating agent comprising vitelline protein B capable of releasing the Brucella strain at a predetermined rate;
• and
• an optional adjuvant or a pharmaceutically acceptable carrier.