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US 2021/0380996 A1Published application
Microorganisms engineered to use unconventional sources of nitrogen
AssigneeGinkgo Bioworks, Inc.
InventorsColin R. South, Arthur J. Shaw, IV
Life of this patent
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Abstract
Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.
Claims7
1-27. (canceled)
A fermentation method of culturing a cell comprising:
- providing a cell comprising a non-native nucleic acid molecule encoding a cyanamide hydratase enzyme;
- contacting the cell with a substrate, wherein the substrate comprises a nitrogen-containing fraction and a non-nitrogen-containing fraction;
- the nitrogen-containing fraction comprises, in an amount from about 10% by weight to about 100% by weight, cyanamide, or a salt thereof;
- and
- culturing the cell, wherein the cell can, unlike a cell of the same species that lacks the nucleic acid molecule, metabolize the cyanamide.
2 more elements
Dependencies are inferred from the claim text.