Versatile display scaffolds for proteins
AssigneeThe Penn State Research Foundation
InventorsScott Eugene Lindner, Susan Hafenstein
Life of this patent
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Abstract
Provided are processes and materials for solving biological or structural information about proteins or other organic molecules. The processes capitalize on a rigid multimeric nanocage formed from self-assembling substructure proteins. The processes and materials allow for recognition and tight, optionally covalent, bonding of any protein molecule with a tag complementary to a capture sequence on the nanocage. The processes and materials may be used to obtain biological or structural information by cryo-electron microscopy and overcome prior limitations of target protein size or salt concentration.
Claims21
-27. (canceled)
A process of immunizing a subject, comprising administering to the subject a three dimensional protein structure comprising:
- a multimeric self-assembling nanocage, the nanocage comprising a plurality of protein substructures, one or more of the protein substructures comprising a linker and a capture sequence on an N-terminus;
- and
- a target protein, the target protein comprising a tag, the tag complementary to the capture sequence to that an association between the tag and the capture sequence covalently bonds said target protein to said protein substructure, or non-covalently bonds said target protein to said protein substructure with a KD or 1012 M or lower.
Dependencies are inferred from the claim text.