Methods and compositions for protein sequencing
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Manjula Pandey
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Abstract
Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides amino acid recognition molecules, such as amino acid binding proteins and fusion polypeptides thereof. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
Claims31
A recombinant amino acid binding protein having an amino acid sequence that is at least 80% identical to a sequence selected from Table 1 or Table 2 and comprising one or more labels.
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An amino acid recognition molecule comprising a polypeptide having at least a first amino acid binding protein and a second amino acid binding protein joined end-to-end, wherein the first and second amino acid binding proteins are separated by a linker comprising at least two amino acids.
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An amino acid recognition molecule comprising a polypeptide having an amino acid binding protein and a labeled protein joined end-to-end, wherein the amino acid binding protein and the labeled protein are separated by a linker comprising at least two amino acids.
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Dependencies are inferred from the claim text.