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US 2021/0363544 A1Published application
Vector-free intracellular delivery by reversible permeabilisation
AssigneeAvectas Limited
InventorsMichael Maguire, Shirley O'Dea
Life of this patent
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Abstract
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
Claims53
-20. (canceled)
A system comprising:
- a housing configured to receive a plate comprising a well;
- a differential pressure applicator configured to apply a differential pressure to the well;
- a delivery solution applicator configured to deliver atomized delivery solution to the well;
- and
- a culture medium applicator configured to deliver a culture medium to the well.
2 more elements
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-43. (canceled)
-57. (canceled)
A method comprising:
- providing a population of cells within a well of a system including a housing configured to receive a plate comprising the well, a differential pressure applicator configured to apply a differential pressure to the well, a delivery solution applicator configured to deliver atomized delivery solution to the well, and a culture medium applicator configured to deliver a culture medium to the well, and
- contacting the population of cells with a volume of aqueous solution.
Dependencies are inferred from the claim text.