Nanoparticle formulations and methods of use for alpha connexin c-terminal peptides
AssigneeFirststring Research, Inc.
InventorsChristina L. GREK, Gautam S. GHATNEKAR
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Abstract
The present disclosure relates to nanoparticle formulations and methods of use for alpha connexin c-terminal peptides. Methods of making and methods of use, for example in the treatment of cancer, are also provided.
Claims77
A composition comprising one or more nanoparticles, wherein the nanoparticles comprise one or more biodegradable or biocompatible polymers and a therapeutically effective amount of a peptide comprising an amino acid sequence according to SEQ ID NO:
- 1.
The nanoparticle composition of any one of the preceding claims, wherein the average diameter of the nanoparticles is between about 10 nm and about 1000 nm.
The nanoparticle composition of any one of the preceding claims, further comprising a cryoprotectant.
The nanoparticle composition of any one of the preceding claims, wherein the nanoparticles have a surface charge characterized by a zeta potential of between about 0 mV to about −30 mV.
The nanoparticle composition of any one of the preceding claims, wherein the nanoparticles have a polydispersity index (PDI) of from about 0.120 to about 0.350.
The nanoparticle composition of any one of the preceding claims, wherein the nanoparticles further comprise zinc oxide or calcium.
The nanoparticle composition of any one of the preceding claims, wherein the nanoparticles have a peptide load greater than about 10 ng peptide/μg.
The nanoparticle composition of any of the preceding claims, wherein the nanoparticles exhibit a controlled peptide release profile substantially corresponding to the following pattern:
- after 24 hours, from about 5% and 60% of the total encapsulated peptide is released;
- and
- after 21 days, from about 50% to 100% of the total encapsulated peptide is released.
A method of manufacturing a topical formulation comprising:
- a) mixing propylene glycol, glycerin, methylparaben and propylparaben until the parabens are completely dissolved;
- b) separately mixing purified water, EDTA, monobasic sodium phosphate, dibasic sodium phosphate and D-mannitol until a clear solution is obtained;
- c) adding the solution from a) to the solution from b), rinsing the container of the solution from a) with purified water, adding the rinse to the combined solutions, and mixing until the combined solutions are visually homogeneous;
- d) with homogenization mixing, adding hydroxyethyl cellulose into the combined solutions of c) and mixing until the polymer is fully dispersed;
- e) separately mixing purified water with an amino acid sequence according to SEQ ID NO:
- 1 or SEQ ID NO:
- 2 until the peptide is completely dissolved;
- f) adding the solution from e) to the solution of d), rinsing the container of the solution from e) with purified water, adding the rinse to the combined solutions, and mixing until the combined solution are homogeneous.
Dependencies are inferred from the claim text.