Plant Vectors, Compositions and Uses Relating Thereto
AssigneeUniversity of Maryland, College Park
InventorAnne Elizabeth Simon
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Abstract
The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of capable of systemic and phloem-limited movement and replication within the host plant.
Claims21
A plus-sense single stranded ribonucleic acid (RNA) vector comprising a heterologous segment(s), wherein said heterologous element(s) is attached to the RNA vector through a lock and dock structure.
An iRNA-based vector having a heterologous segment(s) comprises an siRNA effective against a plant pathogenic bacteria.
An iRNA-based vector comprising a heterologous element comprising a hairpin having a sequence on one side complementary to a sequence within Citrus tristeza virus (CTV).
A plant comprising a sour orange rootstock and an iRNA-based vector comprising a heterologous element that targets a nucleic acid of Citrus tristeza virus (CTV).
A method for introducing a heterologous segment(s) into a host plant comprising introducing into said host plant an iRNA-based vector after a) encapsidating the iRNA vector in a capsid protein other than the capsid protein of CVEV, or b) by agroinfiltration after inoculating an agroinfiltration site with Xanthomonas citri subsp. citri (Xcc).
An iRNA-based vector comprising one or more inserts at one or more of positions 2250, 2301, 2319, 2330, 2331, 2336 and 2083 of a CYVaV based RNA.
A method of making a plus-sense single stranded ribonucleic acid (RNA) vector comprising stabilizing the 3′ UTR structure of a parental construct and inserting one or more destabilizing heterologous segment(s) into the stabilized parental construct.
Dependencies are inferred from the claim text.