Modified gene sequences encoding choline oxidase and a method for preparing betaine usng the same
AssigneeEnzibeta Biotech Private Limited
InventorPRASHANT GAURNG
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Abstract
The present invention provides at least two modified gene sequences, Sequence 1 comprising of nucleotide sequence of SEQ. ID no.1, and Sequence 2 comprising of nucleotide sequence of SEQ. ID no.2, encoding the enzyme choline oxidase wherein the gene sequences have been obtained by modifying the codA gene (Accession no. X84895) encoding choline oxidase from Arthrobacter globiformis, and a method to enzymatically produce betaine using choline oxidases encoded by Sequence 1, and Sequence 2, wherein the enzymatically produced betaine has minimal undesired trimethylamine contamination.
Claims10
1) A modified gene sequence of codA gene comprising a nucleotide sequence of SEQUENCE ID no.1 or SEQUENCE ID no. 2 for encoding choline oxidase;
- wherein
- the choline oxidase encoded by said nucleotide sequence has stability in the pH range 7.0 to 9.0;
- the choline oxidase encoded by said nucleotide sequence has stability at a temperature range of 10-50° C.;
- the choline oxidase encoded by said nucleotide sequence has stability at a temperature range of 10-50° C. for up to 120 minutes;
- and
- the choline oxidase thus encoded is useful to produce betaine from choline.
4) A method for producing betaine from choline comprising the steps of:
- a) making a reaction mixture comprising of choline aqueous solution, an enzyme and optionally hydrogen peroxide catalyzing agents;
- b) carrying out a reaction using the reaction mixture in a reactor;
- and
- c) on completion of the reaction, separating the enzyme from the mixture;
- wherein, the enzyme comprises choline oxidase encoded by nucleotide sequence of SEQUENCE ID no.1 or SEQUENCE ID no.2;
- and
- the method has choline to betaine conversion efficiency of 1:1 molar ratio.
Dependencies are inferred from the claim text.