Methods of producing plant protein from food waste using microalgae
AssigneeSophie's BioNutrients Pte. Ltd.
InventorYao-Hsin Wang
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Abstract
Methods to extract chlorella protein from algae powder are described. A first extraction method is an alkaline solution extraction method. A second extraction method is an enzyme extraction method. A third method is a low-temperature deep eutectic solvents (DES) extraction method. According to each of these methods, a protein recovery rate is calculated from a protein extract solution of chlorella protein.
Claims20
An extraction method for chlorella protein, the method comprising:
- adding an alkaline solution to algae powder to form a mixture;
- extracting the chlorella protein from the mixture at a temperature for a first time period;
- centrifuging the mixture for a second time period to obtain a protein extract solution of the chlorella protein;
- and
- calculating a protein recovery rate from the protein extract solution of the chlorella protein.
An enzyme extraction method for chlorella protein, the method comprising:
- dissolving an algae powder in water to form a solution;
- adding an alkaline protease to the solution;
- adjusting a pH of the solution to the pH of 8.0;
- hydrolyzing the solution at a temperature with an alkaline solution for a first time period;
- centrifuging the mixture for a second time period to obtain a protein extract solution of the chlorella protein;
- and
- calculating a protein recovery rate from the protein extract solution of the chlorella protein.
A low-temperature deep eutectic solvent (DES) extraction method of chlorella protein, the method comprising:
- adding a first material:a second material having molar ratios of 1:2 to an algae powder:cryogenic co-melt solvent having molar ratios of 1:9 to form a mixture;
- reacting the mixture at a temperature for a first time period;
- centrifuging the mixture for a second time period to obtain a protein extract solution of the chlorella protein;
- and
- calculating a protein recovery rate from the protein extract solution of the chlorella protein.
Dependencies are inferred from the claim text.