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AssigneeIntrexon Corporation
InventorsBela Krisztian Szamecz, Szilvia Varszegi, Attila Nemeth, Lorand Szabo, Abhunav Kumar
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Disclosed herein are microorganism and methods that can be used for the synthesis of cannabigerolic acid (CBGA) and cannabinoids. The methods disclosed can be used to produce CBGA, Δ9-tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabichromenic acid (CBCA), Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabichromene (CBC). Enzymes useful for the synthesis of CBGA and cannabinoids, include but are not limited to acyl activating enzyme (AAE1), polyketide synthase (PKS), olivetolic acid cyclase (OAC), prenyltransferase (PT), THCA synthase (THCAS), CBDA synthase (CBDAS), CBCA synthase (CBCAS), HMG-Co reductase (HMG1), and/or farnesyl pyrophosphate synthetase (ERG20). The microorganisms can also have one or more genes disrupted, such as gene that that controls beta oxidation of long chain fatty acids.
A genetically modified microorganism comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
A genetically modified microorganism comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
A genetically modified microorganism comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
A genetically modified microorganism comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
A method of making CBGA comprising:
A method of making CBGA comprising:
A method of making CBGA comprising:
A method of making CBGA comprising:
A method of making a cannabinoid comprising:
A method of making a cannabinoid comprising:
A method of making a cannabinoid comprising:
A method of making a cannabinoid comprising:
A vector comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
A vector comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
A vector comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
A vector comprising a polynucleotide that is at least 60% identical to SEQ ID NO:
An isolated polynucleotide comprising a nucleotide sequence that is at least 60% identical to SEQ ID NO:
An isolated polynucleotide comprising a nucleotide sequence that is at least 60% identical to SEQ ID NO:
An isolated polynucleotide comprising a nucleotide sequence that is at least 60% identical to SEQ ID NO:
An isolated polynucleotide comprising a nucleotide sequence that is at least 60% identical to SEQ ID NO:
A method of making a genetically modified microorganism capable of synthesizing CBGA comprising:
A method of making a genetically modified microorganism capable of synthesizing CBGA comprising:
A method of making a genetically modified microorganism capable of synthesizing CBGA comprising:
A method of making a genetically modified microorganism capable of synthesizing CBGA comprising:
A method of making a genetically modified microorganism capable of synthesizing cannabinoid comprising:
A method of making a genetically modified microorganism capable of synthesizing cannabinoid comprising:
A method of making a genetically modified microorganism capable of synthesizing cannabinoid comprising:
A method of making a genetically modified microorganism capable of synthesizing cannabinoid comprising:
A genetically modified microorganism capable of making a CBGA comprising a disruption of an endogenous gene that is FOX1.
Dependencies are inferred from the claim text.