Enzymatic compositions for carbohydrate antigen cleavage on donor organs, methods and uses associated therewith
AssigneesThe University of British Columbia, University Health Network
InventorsMarcelo CYPEL, Aizhou WANG, Shafique KESHAVJEE, Stephen G. WITHERS, Peter RAHFELD, Jayachandran KIZHAKKEDATHU
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Abstract
Provided herein are perfusion fluids for enzymatically cleaving A-antigens from a donor organ, and methods, uses, associated therewith. In particular, the perfusion fluids comprise two enzymes, GalNAcDeacetylase and Galactosaminidase and the fluids may further comprise a buffered extracellular solution and/or a crowing agent. Furthermore, the compositions described herein were found to have activity at temperatures and pH levels suitable for cell viability.
Claims25
A perfusion fluid for enzymatically cleaving A-antigens from a donor organ comprising:
- (a) a purified GalNAcDeacetylase protein;
- and
- (b) a purified Galactosaminidase protein.
A method for enzymatically cleaving A-antigens ex vivo from a donor organ, the method comprising:
- (a) perfusing a donor organ displaying type A antigen with a fluid comprising GalNAcDeacetylase protein and a Galactosaminidase protein for a period of time sufficient to allow the enzymes to cleave A-antigens from the donor organ;
- or
- (b) incubating a donor organ displaying type A antigen with a fluid comprising GalNAcDeacetylase protein and a Galactosaminidase protein for a period of time sufficient to allow the enzymes to cleave A-antigens from the donor organ.
Dependencies are inferred from the claim text.