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US 2021/0340603 A1Published application
Dna adductomics by mass tag prelabeling
AssigneeNortheastern University
InventorsRoger W. Giese, Poguang Wang
Life of this patent
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Abstract
Disclosed is a method for detecting the presence of a DNA adduct in DNA. The method involves the pre-labeling of an adducted nucleotide in the DNA with a quaternary ammonium compound.
Claims26
A method for detecting the presence of a DNA adduct in DNA, comprising the following steps:
- (1) reacting an adducted nucleotide in the DNA with a labeling precursor L1 to provide an adducted nucleotide labeled with label L;
- (2) enzymatically digesting the DNA to provide a free adducted nucleotide comprising the label L;
- and
- (3) detecting the free adducted nucleotide comprising the label L by mass spectrometry,
- wherein the label L comprises a quaternary amine group.
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A method for detecting the presence of a DNA adduct in DNA, comprising the following steps:
- (1) reacting an adducted nucleotide in the DNA with a labeling precursor L1 to provide an adducted nucleotide labeled with label L;
- (2) enzymatically digesting the DNA to provide a free adducted nucleotide comprising the label L;
- (3) subjecting the free adducted nucleotide comprising the label L to conditions sufficient to provide the corresponding nucleoside comprising the label L;
- and
- (4) detecting the nucleoside comprising the label L by mass spectrometry,
- wherein the label L comprises a quaternary amine group, or
- for detecting the presence of a DNA adduct in DNA, comprising the following steps:
- (1) reacting an adducted nucleotide in the DNA with a labeling precursor L1 to provide an adducted nucleotide labeled with label L;
- (2) enzymatically digesting the DNA to provide a free adducted nucleotide comprising the label L;
- (3) subjecting the free adducted nucleotide comprising the label L to conditions sufficient to provide the corresponding nucleoside comprising the label L;
- (4) subjecting the nucleoside comprising the label L to conditions sufficient to provide the corresponding nucleobase comprising the label L;
- and
- (5) detecting the nucleobase comprising the label L by mass spectrometry,
- wherein the label L comprises a quaternary amine group.
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A method for detecting the presence of a DNA adduct in DNA, comprising the following steps:
- (1) reacting an adducted nucleobase in the DNA with a labeling precursor L1 to provide an adducted nucleobase labeled with label L;
- (2) subjecting the DNA to neutral thermal hydrolysis to provide a free adducted nucleobase labeled with label L;
- and
- (3) detecting the free adducted nucleobase labeled with label L by mass spectrometry,
- wherein the label L comprises a quaternary amine group.
2 more elements
Dependencies are inferred from the claim text.