# US 2021/0340595 A1 · Blood Sample Processing and Nucleic Acid Amplification Systems, Devices, and Methods > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0340595 A1 - Document ID: US20210340595A1 - Status: Published application - Kind: A1 - Application number: 17/210,083 - Filed: 2021-03-23 - Published: 2021-11-04 - Earliest claimed: 2018-09-25 - Assignees: The Trustees of Columbia University in the City of New York - Applicants: The Trustees of Columbia University in the City of New York - Inventors: Samuel K. SIA; Nicole R. BLUMENFELD; Rodrigo C. CHAVES; Giuseppe MODICA; Michael LUETOLF - Web page: https://patentgrep.com/patent/US20210340595A1 ## CPC - C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) - B01L 3/563: {Joints or fittings; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors} - B01L 3/5635: {connecting two containers face to face, e.g. comprising a filter} - C12N 15/1017: {by filtration, e.g. using filters, frits, membranes} - B01L 2200/027: for microfluidic devices - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract Aspects described herein provide blood sample purification and microfluidic nucleic acid amplification systems and methods for purifying blood samples and amplifying a target nucleic acid on the same system. The described systems and methods include a filter component and a microfluidic amplification component for amplification of nucleic acid from the blood sample, and can be used, for example, for point of care blood analysis. ## Claims ### Claim 1 A blood sample preparation system comprising: a filter component comprising at least a first filter and a second filter for preparing a blood sample, each filter having a different pore size; and a microfluidic amplification component for amplification of nucleic acid from the blood sample. ### Claim 2 (depends on 1) The blood sample preparation system of claim 1, wherein a volume of the blood sample is greater than about 100 μl. ### Claim 3 (depends on 1) The blood sample preparation system of claim 1, wherein the microfluidic amplification component is in fluid connection with the filter component. ### Claim 4 (depends on 1) The blood sample preparation system of claim 1, further comprising a DNA purification component. ### Claim 5 (depends on 1) The blood sample preparation system of claim 1, wherein the filter component further comprises: an inlet component having an inlet port for receiving a blood sample; an outlet component having an outlet port for releasing a filtered blood sample; and a filter support for retaining a first filter and a second filter. ### Claim 6 (depends on 5) The blood sample preparation system of claim 5, wherein the first filter and second filter have a pore size, and the pore size of the first filter is larger than the pore size of the second filter. ### Claim 7 (depends on 6) The blood sample preparation system of claim 6, wherein the pore size of the first filter and the second filter range from about 1 μm to about 25 μm. ### Claim 8 (depends on 1) The blood sample preparation system of claim 1, wherein the microfluidic amplification component comprises a polymerase chain reaction chamber for amplifying nucleic acid. ### Claim 9 (depends on 8) The blood sample preparation system of claim 8, wherein the microfluidic amplification component further comprises at least two inlets for the polymerase chain reaction chamber for providing reagents to the polymerase chain reaction chamber. ### Claim 10 (depends on 9) The blood sample preparation system of claim 9, wherein the microfluidic amplification component further comprises an extraction chamber disposed between the at least two inlets and the polymerase chain reaction chamber for extracting nucleic acid from the blood sample. ### Claim 11 (depends on 8) The blood sample preparation system of claim 8, wherein the microfluidic amplification component further comprises an outlet from the polymerase chain reaction chamber for disposal of waste products. ### Claim 12 (depends on 8) The blood sample preparation system of claim 8, wherein the microfluidic amplification component further comprises at least one valve for controlling flow of reagents to and from the polymerase chain reaction chamber. ### Claim 13 (depends on 12) The blood sample preparation system of claim 12, wherein the at least one valve is a solenoid valve. ### Claim 14 (depends on 13) The blood sample preparation system of claim 13, wherein the solenoid valve comprises a sensing tip. ### Claim 15 (depends on 8) The blood sample preparation system of claim 8, wherein the microfluidic amplification component further comprises an actuator. ### Claim 16 (depends on 15) The blood sample preparation system of claim 15, wherein the actuator comprises a membrane. ### Claim 17 A method of filtering and amplifying a nucleic acid target from a blood sample, comprising: providing a blood sample to a filtering component; filtering the blood sample through a first filter and a second filter to form a filtered blood sample; and amplifying the nucleic acid target from the filtered blood sample. ### Claim 18 (depends on 17) The method of claim 17, wherein the first filter and the second filter have a pore size, and the pore size of the first filter is larger than the pore size of the second filter. ### Claim 19 (depends on 18) The method of claim 18, wherein the pore size of the first filter and the second filter range from about 1 μm to about 25 μm. ### Claim 20 A method of filtering a blood sample and amplifying a nucleic acid target from the blood sample, comprising: providing a blood sample to a filtering component; filtering the blood sample through a first filter and a second filter to form a filtered blood sample; purifying nucleic acid from the filtered blood sample to form a purified nucleic acid sample; and amplifying the nucleic acid target from the purified nucleic sample.