# US 2021/0340559 A1 · Gene for induction of parthenogenesis, a component of apomictic reproduction

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- Number: US 2021/0340559 A1
- Document ID: US20210340559A1
- Status: Published application
- Kind: A1
- Application number: 16/821,755
- Filed: 2020-03-17
- Published: 2021-11-04
- Earliest claimed: 2013-10-21
- Assignees: University of Georgia Research Foundation, Inc.
- Applicants: University of Georgia Research Foundation, Inc.
- Inventors: Peggy Ozias-Akins; Joann A. Conner
- Web page: https://patentgrep.com/patent/US20210340559A1

## CPC

- C12N 15/8287: {for fertility modification, e.g. apomixis}
- A01H 5/08: Fruits
- A01H 6/346: {Cucumis sativus[cucumber]}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

Methods and compositions disclosed herein generally relate to genes involved in plant reproduction and methods of using the same.

## Claims

### Claim 1

A method of propagating one or more gametophytic or sporophytic cells in an ovule of a plant in the absence of egg cell fertilization, the method comprising:
transforming a plant with an ASGR-BBML gene construct comprising a nucleic acid encoding a polypeptide having at least 75% sequence similarity to the polypeptide of SEQ ID NO: 4, wherein the nucleic acid is operably-linked to a promoter;
and
growing and selecting a progeny plant from the one or more gametophytic or sporophytic cells, wherein the progeny plant contains one or more sets of chromosomes from the transformed plant, wherein propagation of the plant occurs in the absence of egg cell fertilization.

### Claim 2 (depends on 1)

The method of claim 1, wherein the ASGR-BBML gene construct further comprises one or more untranslated region (UTR).

### Claim 3 (depends on 2)

The method of claim 2, wherein the ASGR-BBML gene construct further comprising one or more UTR has at least 70% sequence identity to SEQ ID NO: 1 or a fully complementary strand thereof.

### Claim 4

(canceled)

### Claim 5

(canceled)

### Claim 6 (depends on 1)

The method of claim 1, wherein the promoter comprises a nucleotide having at least 70% sequence identity to SEQ ID NO: 5 or a fully complementary strand thereof.

### Claim 7 (depends on 2)

The method of claim 2 wherein the ASGR-BBML gene construct has at least 70% sequence identity to SEQ ID NO: 3 or a fully complementary strand thereof.

### Claim 8 (depends on 1)

The method of claim 1, wherein an embryo is formed from an unreduced egg.

### Claim 9 (depends on 1)

The method of claim 1, wherein an embryo is formed from a reduced egg.

### Claim 10 (depends on 1)

The method of claim 1, wherein an embryo is formed from a somatic cell.

### Claim 11 (depends on 1)

The method of claim 1 in which a polyploid plant is transformed to produce a diploid or dihaploid progeny plant.

### Claim 12 (depends on 1)

The method of claim 1, in which a diploid plant is transformed to produce a haploid progeny plant.

### Claim 13 (depends on 12)

The method of claim 12, in which the haploid progeny plant is treated to achieve chromosome doubling and production of a homozygous plant.

### Claim 14 (depends on 1)

The method of claim 1, wherein the progeny plant is obtained via culturing.

### Claim 15 (depends on 1)

The method of claim 1, wherein the flowering plant is amonocot.

### Claim 16 (depends on 1)

The method of claim 1, wherein the plant is a dicot.

### Claim 17 (depends on 1)

The method of claim 1, wherein the plant comprises a grass or a leguminous plant.

### Claim 18 (depends on 17)

The method of claim 17, wherein the grass is a species of millet, rice, maize, wheat, sorghum, or switchgrass.

### Claim 19 (depends on 1)

The method of claim 1, wherein the plant is heterozygous and is transformed to produce a clonal offspring.

### Claim 20 (depends on 1)

The method of claim 1, wherein the plant is heterozygous and is transformed to produce a haploid offspring.

### Claim 21 (depends on 1)

The method of claim 1, wherein the method is used to propagate one or more heritable traits in the plant.

### Claim 22 (depends on 1)

A plant or plant part produced by the method of claim 1.

### Claim 23

A method of producing a plant capable of being reproduced in the absence of egg cell fertilization, the method comprising:
transforming a plant with an ASGR-BBML gene construct encoding a polypeptide having at least 75% sequence similarity the polypeptide of SEQ ID NO: 4, thereby producing a plant capable of being reproduced in the absence of egg cell fertilization.

### Claim at position 24

24-28. (canceled)