Cells and Methods for the Production of Ursodeoxycholic Acid and Precursors Thereof

Abstract

Genetically-modified cell capable of producing UD CA, cholic acid, and/or another UDCA precursor comprising at least one heterologous polynucleotide encoding an enzyme involved in a metabolic pathway that converts sugar to UDCA, cholic acid, and/or another UDCA precursor. Method of making UDCA, cholic acid, and/or another UDCA precursor using such a cell. Use of UDCA or UDCA precursor produced using such a method for the manufacture of a medicament for the treatment of a disease or symptom of a disease. Medicament comprising UDCA or UDCA precursor made using such a method. Method of treating a disease or symptom of a disease comprising administering UDCA or a UDCA precursor made using such a method. Isolated nucleic acid encoding at least one enzyme involved in a metabolic pathway that converts sugar to UDCA, cholic acid, and/or another UDCA precursor. Vector comprising a nucleic acid encoding at least one enzyme involved in a metabolic pathway that converts sugar to UDCA, cholic acid and/or another UDCA precursor. Method of making a genetically-modified cell capable of synthesizing UDCA, cholic acid, and/or another UDCA precursor. Composition comprising UDCA or a UDCA precursor, a free acid or CoA thereof, or a pharmaceutically-acceptable derivative or prodrug thereof.

Dependencies are inferred from the claim text.