# US 2021/0340490 A1 · Method for enriching a biomass with proteins

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- Number: US 2021/0340490 A1
- Document ID: US20210340490A1
- Status: Published application
- Kind: A1
- Application number: 17/273,678
- Filed: 2019-09-05
- Published: 2021-11-04
- Earliest claimed: 2018-09-05
- Assignees: Fermentalg
- Applicants: Fermentalg
- Inventors: Olivier CAGNAC; Axel ATHANE; Julien DEMOL
- Web page: https://patentgrep.com/patent/US20210340490A1

## CPC

- C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00)
- C12N 1/125: Unicellular algae isolates
- A21D 2/264: {Vegetable proteins}
- A23C 9/1315: {Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products}
- A23C 9/1526: {Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof}
- A23C 19/093: Addition of non-milk fats or non-milk proteins
- A23J 1/008: {from microorganisms (A23J1/18 takes precedence)}
- A23J 1/009: {from unicellular algae (seaweed A23J1/006)}
- A23J 3/20: Proteins from microorganisms or unicellular algae
- A23J 3/227: {Meat-like textured foods (meat extenders A23L13/00)}
- A23J 3/347: {of proteins from microorganisms or unicellular algae}
- A23K 10/12: by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
- A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10)
- A23L 2/39: Dry compositions
- A23L 2/66: Proteins
- A23L 11/05: {Mashed or comminuted pulses or legumes; Products made therefrom (A23L11/30 takes precedence; tofu or soya cheese A23L11/45; soy drinks A23L11/65)}
- A23L 29/281: Proteins, e.g. gelatin or collagen
- A23L 33/195: Proteins from microorganisms
- A61K 36/04: Rhodophycota or rhodophyta (red algae), e.g. Porphyra
- C12N 1/06: Lysis of microorganisms
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- C12R 2001/89: Algae {; Processes using algae}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A21D: TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS (preservation of flour or dough before baking A23B40/00; preservation of bakery products A23B45/00)
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention relates to a method for protein enrichment of a biomass of red unicellular algae such as Galdieria and to the biomass thus obtained.

## Claims

### Claim 1

A process for producing a protein-rich biomass of lysed microorganisms, wherein the process comprises the steps of
b.1. suspending a biomass of lysed microorganisms,
b.2. acidifying the suspension to a pH less than or equal to 5,
c. recovering the lysed biomass fraction insoluble at pH less than or equal to 5, and
if need be, repeating steps b.1., b.2. and c. on the lysed biomass fraction insoluble at pH less than or equal to 5 recovered in c.

### Claim 2 (depends on 1)

The process according to claim 1, wherein the acid suspension obtained in b.2. has a pH less than or equal to 4.5.

### Claim 3 (depends on 1)

The process according to claim 1, wherein the acid suspension obtained by step b.2. has a dry matter content of 1% to 30%.

### Claim 4 (depends on 1)

The process according to claim 1, wherein steps b.1. and b.2. are consecutive or simultaneous.

### Claim 5 (depends on 1)

The process according to claim 1, wherein steps b.1. and b.2. are consecutive, step b.2. being carried out directly after step b.1. without any further biomass treatment step, in particular without a defatting step.

### Claim 6 (depends on 1)

The process according to claim 1, wherein the process comprises a preliminary step a. of lysis of a biomass of previously cultivated microorganisms.

### Claim 7 (depends on 1)

The process according to claim 1, wherein the biomass recovered in c. is dried.

### Claim 8 (depends on 1)

The process according to claim 1, wherein the microorganisms are selected from unicellular red algae (URA) of the families Cyanidiaceae or Galdieriaceae.

### Claim 9 (depends on 1)

Protein-rich lysed microorganism biomass obtainable by the process according to claim 1.

### Claim 10 (depends on 9)

The biomass according to claim 9, wherein the said biomass comprises at least 60% proteins in relation to the dry matter, and at least a total sugar content of less than 20% in relation to the dry matter and/or a glycogen content of less than 10% in relation to the dry matter and/or a fat content of at least 5% in relation to the dry matter.

### Claim 11 (depends on 9)

Food or food composition for human or animal consumption wherein the said food or food composition comprises a biomass of lysed microorganisms as defined in claim 9.

### Claim at position 12

-13. (canceled)

### Claim 14

A process for producing a protein-rich biomass of lysed microorganisms, wherein the process comprises the steps of:
a. lysis of a biomass of microorganisms,
b.1. suspending the biomass of lysed microorganisms,
b.2. acidifying the suspension to a pH less than or equal to 5,
c. recovering the lysed biomass fraction insoluble at pH less than or equal to 5, and
if need be, repeating steps b.1., b.2. and c. on the lysed biomass fraction insoluble at pH less than or equal to 5 recovered in c.

### Claim 15 (depends on 14)

The process according to claim 14, wherein the acid suspension obtained in b.2. has a pH less than or equal to 4.5.

### Claim 16 (depends on 14)

The process according to claim 14, wherein the acid suspension obtained by step b.2. has a dry matter content of 1% to 30%.

### Claim 17 (depends on 1)

The process according to claim 1, wherein the microorganisms are selected from unicellular red algae (URA) of the genera Cyanidioschyzon, Cyanidium or Galdieria.

### Claim 18 (depends on 1)

The process according to claim 1, wherein the microorganisms are selected from unicellular red algae (URA) of the species Cyanidioschyzon merolae 10D, Cyanidioschyzon merolae DBV201, Cyanidium caldarium, Cyanidium daedalum, Cyanidium maximum, Cyanidium partitum, Cyanidium rumpens, Galdieria daedala, Galdieria maxima, Galdieria partita or Galdieria sulphuraria.