# US 2021/0340484 A1 · Device for a cell suspension culture > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0340484 A1 - Document ID: US20210340484A1 - Status: Published application - Kind: A1 - Application number: 17/269,649 - Filed: 2019-10-28 - Published: 2021-11-04 - Earliest claimed: 2018-11-09 - Assignees: Cellex, Inc. - Applicants: Cellex S.R.L. - Inventors: Giuseppe FALVO D'URSO LABATE - Web page: https://patentgrep.com/patent/US20210340484A1 ## CPC - C12M 35/04: {Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli} - C12M 23/02: {Form or structure of the vessel (large containers B65D88/00)} - C12M 27/18: {Flow directing inserts} - C12M 41/32: {of substances in solution} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract The device for a cell suspension culture comprises a culture chamber comprising first and second axisymmetric-shaped walls having at least a partially curved radial section the concavity thereof facing the inside of the culture chamber, the walls delimiting an inner compartment and form an inner continuous surface; an inlet opening provided in the first wall and adapted to enable a fluid to enter; a supplying conduit connected to the inlet opening; and a valve member disposed inside the culture chamber adapted to tightly close or open the inlet opening. The device comprises also a device for moving the valve member, disposed outside the culture chamber. ## Claims ### Claim 1 A device for a cell suspension culture comprising: a culture chamber comprising a first wall and a second wall delimiting an inner compartment said first and second walls having an axisymmetric shape and being connected to each other in order to form an inner continuous surface; an inlet opening provided in the first wall and suitable to enable a fluid to enter the culture chamber; a supply conduit connected to the inlet opening and suitable to enable to supply the fluid towards the culture chamber through the inlet opening; a valve member arranged inside the culture chamber at the inlet opening, displaceable between an open position, wherein the valve member is distanced from the inlet opening enables the outflow of the fluid inside the culture chamber, and a closed position wherein the valve member tightly closes the inlet opening; and a movement device for moving the valve member, suitable to cause the valve member to be displaced between the open position and the closed position, said movement device being disposed outside the culture chamber. ### Claim 2 (depends on 1) The device according to claim 1, further comprising a base body inside which one of the first and second walls is arranged, and a lid suitable to be tightly connected to the base body and in which the other one of the first and second walls of the culture chamber is arranged. ### Claim 3 (depends on 1) The device according to claim 1, wherein the first wall comprises a bottom zone and a joint zone suitable to connect to each other the bottom zone and the second wall wherein the joint zone has an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber. ### Claim 4 (depends on 1) The device according to claim 1, wherein the second wall comprises a central zone and an annular zone with an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber. ### Claim 5 (depends on 1) The device according to claim 1, wherein in the open position, the valve member opens a port for the passage of the fluid, with a predetermined height with respect to the first wall. ### Claim 6 (depends on 3) The device according to claim 3, wherein the cross-section of the joint zone of the first wall has a curve radius comprised between five and twenty times the height of the port of the fluid passage. ### Claim 7 (depends on 4) The device according to claim 4, wherein the cross-section of the annular zone of the second wall has a curve radius comprised between three and ten times the height of the port for the passage of the fluid, and is less than the curve radius of the joining zone of the first wall. ### Claim 8 (depends on 1) The device according to claim 1, further comprising an outlet opening for enabling the fluid to exit the culture chamber, said outlet opening being arranged in the second wall. ### Claim 9 (depends on 1) The device according to claim 1, wherein the movement device of the valve member comprises a magnetic element, the valve member being provided with a magnetic counter-element suitable to interact with said magnetic element. ### Claim 10 (depends on 1) The device according to claim 1, comprising a membrane made of an elastically deformable material and arranged outside the first wall of the culture chamber, said membrane being arranged between the first wall and the movement device, and wherein the movement device mechanically interacts with the valve member through said membrane. ### Claim 11 A device for a cell suspension culture comprising: a culture chamber comprising a first wall and a second wall delimiting an inner compartment, said first and second walls having an axisymmetric shape and being connected to each other in order to form an inner continuous surface; an inlet opening provided in the first wall and suitable to enable a fluid to enter the culture chamber; a supply conduit connected to the inlet opening and suitable to enable to supply the fluid towards the culture chamber through the inlet opening; a valve member arranged inside the culture chamber at the inlet opening, displaceable between an open position, wherein the valve member is distanced from the inlet opening and enables the outflow of the fluid inside the culture chamber, and a dosed position wherein the valve member tightly doses the inlet opening; and a base body inside which one of the first and second walls is arranged, and a lid suitable to be tightly connected to the base body and in which the other one of the first and second walls of the culture chamber is arranged; wherein the first wall comprises a bottom zone and a joint zone suitable to connect to each other the bottom zone and the second wall, wherein the joint zone has an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber; wherein the second wall comprises a central zone and an annular zone with an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber; wherein the second wall comprises a central zone and an annular zone with an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber; and wherein in the open position, the valve member opens a port for the passage of the fluid, with a predetermined height with respect to the first wall ### Claim 12 (depends on 11) The device according to claim 11, further comprising a movement device for moving the valve member, suitable to cause the valve member to be displaced between the open position and the dosed position, said movement device being disposed outside the culture chamber; ### Claim 13 (depends on 11) The device according to claim 11, wherein the cross-section of the joint zone of the first wall has a curve radius comprised between five and twenty times the height of the port of the fluid passage. ### Claim 14 (depends on 13) The device according to claim 13, wherein the cross-section of the annular zone of the second wall has a curve radius comprised between three and ten times the height of the port for the passage of the fluid, and is less than the curve radius of the joining zone of the first wall. ### Claim 15 (depends on 11) The device according to claim 11, further comprising an out let opening for enabling the fluid to exit the culture chamber, said outlet opening being arranged in the second wall. ### Claim 16 (depends on 12) The device according to claim 12, wherein the movement device of the valve member comprises a magnetic element, the valve member being provided with a magnetic counter-element suitable to interact with said magnetic element. ### Claim 17 (depends on 16) The device according to claim 16, comprising a membrane made of an elastically deformable material and arranged outside the first wall of the culture chamber, said membrane being arranged between the first wall and the movement device, and wherein the movement device mechanically interacts with the valve member through said membrane. ### Claim 18 A device for a cell suspension culture comprising: a culture chamber comprising a first wall and a second wall delimiting an inner compartment, said first and second walls having an axisymmetric shape and being connected to each other in order to form an inner continuous surface; an inlet opening provided in the first wall and suitable to enable a fluid to enter the culture chamber; a supply conduit connected to the inlet opening and suitable to enable to supply the fluid towards the culture chamber through the inlet opening; a valve member arranged inside the culture chamber at the inlet opening, displaceable between an open position, wherein the valve member is distanced from the inlet opening and enables the outflow of the fluid inside the culture chamber, and a dosed position wherein the valve member tightly doses the inlet opening; a movement device for moving the valve member, suitable to cause the valve member to be displaced between the open position and the dosed position, said movement device being disposed outside the culture chamber; and an outlet opening for enabling the fluid to exit the culture chamber, said outlet opening being arranged in the second wall; wherein the first wall comprises a bottom zone and a joint zone suitable to connect to each other the bottom zone and the second wall, wherein the joint zone has an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber; wherein the second wall comprises a central zone and an annular zone with an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber; wherein in the open position, the valve member opens a port for the passage of the fluid, with a predetermined height with respect to the first wall; wherein the cross-section of the joint zone of the first wall has a curve radius comprised between five and twenty times the height of the port of the fluid passage; wherein the cross-section of the annular zone of the second wall has a curve radius comprised between three and ten times the height of the port for the passage of the fluid, and is less than the curve radius of the joining zone of the first wall; and wherein the movement device of the valve member comprises a magnetic element, the valve member being provided with a magnetic counter-element suitable to interact with said magnetic element. ### Claim 19 (depends on 18) The device according to claim 18, further comprising a base body inside which one of the first and second walls is arranged, and a lid suitable to be tightly connected to the base body and in which the other one of the first and second wads of the culture chamber is arranged. ### Claim 20 (depends on 18) The device according to claim 18, comprising a membrane made of an elastically deformable material and arranged outside the first wall of the culture chamber, said membrane being arranged between the first wall and the movement device, and wherein the movement device mechanically interacts with the valve member through said membrane.