# US 2021/0340476 A1 · Container with embedded structure > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0340476 A1 - Document ID: US20210340476A1 - Status: Published application - Kind: A1 - Application number: 17/286,452 - Filed: 2019-02-22 - Published: 2021-11-04 - Earliest claimed: 2019-02-22 - Assignees: Origem Biotechnology Inc. - Applicants: Origem Biotechnology Inc. - Inventors: Hao Chen CHANG; Hung Jui CHEN - Web page: https://patentgrep.com/patent/US20210340476A1 ## CPC - C12M 23/04: {Flat or tray type, drawers (C12M23/10, C12M23/12, C12M23/16 take precedence)} - C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)} - B65D 75/28: Articles or materials wholly enclosed in composite wrappers, i.e. wrappers formed by associating or interconnecting two or more sheets or blanks - C12M 23/26: {flexible (flexible containers for laboratory use B01L3/505)} - C12M 23/38: {Caps; Covers; Plugs; Pouring means} - C12M 25/06: {Plates; Walls; Drawers; Multilayer plates} - C12N 5/00: Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - B65D: CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A container with an embedded structure mainly includes: a first plate with a first plate body, a first end surface located on one side of the first plate body; a second plate with a second plate body, a second end surface opposite to the first end surface and located on one side of the second plate body, a flexible protrusion is annularly protruded on the second end surface and abuts against the first plate to form an accommodating space between the first end surface, the second end surface, and the protrusion for a fluid to flow in the accommodating space, and the accommodating space has an accommodating height between the first end surface and the second end surface; and a shape of a portion of a free end of the protrusion abutting the first end surface is deformed. ## Claims ### Claim 1 A container with an embedded structure comprising: a first plate with a first plate body, a first end surface located on one side of the first plate body; a second plate with a second plate body, a second end surface opposite to the first end surface and located on one side of the second plate body, a flexible protrusion being annularly protruded on the second end surface and abutting against the first plate to form an accommodating space between the first end surface, the second end surface, and the protrusion for a fluid to flow in the accommodating space, and the accommodating space having an accommodating height between the first end surface and the second end surface; and a shape of a portion of a free end of the protrusion abutting the first end surface being deformed. ### Claim 2 (depends on 1) The container with the embedded structure as claimed in claim 1, wherein the first plate further includes a concave portion complementarily embedded with the protrusion, and recessed with a predetermined depth from the first end surface toward an inner direction of the first plate body. ### Claim 3 (depends on 2) The container with the embedded structure as claimed in claim 2, wherein the protrusion further includes a first convex body inserted in the concave portion, a free end of the first convex body abuts against a bottom surface of the concave portion, a height of the first convex body protruding outside of the second end surface is greater than a depth of the concave portion, and a portion of the first convex body exposed outside of the concave portion constitutes the accommodating height. ### Claim 4 (depends on 2) The container with the embedded structure as claimed in claim 2, wherein the protrusion further includes a first convex body inserted in the concave portion, and a second convex body disposed on one side of the first convex body, a height of the first convex body protruding outside of the second end surface is greater than a height of the second convex body protruding outside of the second end surface, and a stopping distance between the free end of the first convex body and a free end of the second convex body is smaller than the depth of the concave portion, so that the free end of the second convex body abuts on the first end surface, and a height of the second convex body constitutes the accommodating height. ### Claim 5 (depends on 2) The container with the embedded structure as claimed in claim 2, wherein the protrusion further includes a first convex body inserted in the concave portion, a height of the convex body protruding from the second end surface is not greater than a depth of the concave portion; the first plate further includes a groove located on the first end surface and surrounded by the concave portion, a predetermined groove depth is recessed from the first end surface toward the inner direction of the first plate body, and the groove depth of the groove constitutes the accommodating height. ### Claim 6 (depends on 2) The container with the embedded structure as claimed in claim 2, wherein the first plate further includes a groove located on the first end surface and surrounded by the concave portion, a predetermined groove depth is recessed from the first end surface toward the inner direction of the first plate body; the protrusion further includes a first convex body inserted in the concave portion, and a second convex body with a predetermined thickness disposed on one side of the first convex body, a height of the first convex body protruding outside of the second end surface is greater than a height of the second convex body protruding outside of the second end surface, and a stopping distance between the free end of the first convex body and the free end of the second convex body is smaller than the depth of the concave portion, so that the free end of the second convex body abuts on the first end surface, and a sum of the groove depth of the groove and the height of the second convex body constitutes the accommodating height. ### Claim 7 (depends on 3) The container with the embedded structure as claimed in any one of claim 3, wherein an axial sectional width of the protrusion along its own annular center is not less than an axial sectional width of the concave portion along its own annular center. ### Claim 8 (depends on 4) The container with the embedded structure as claimed in any one of claim 4, wherein an axial sectional width of the protrusion along its own annular center is not less than an axial sectional width of the concave portion along its own annular center. ### Claim 9 (depends on 5) The container with the embedded structure as claimed in any one of claim 5, wherein an axial sectional width of the protrusion along its own annular center is not less than an axial sectional width of the concave portion along its own annular center. ### Claim 10 (depends on 6) The container with the embedded structure as claimed in any one of claim 6, wherein an axial sectional width of the protrusion along its own annular center is not less than an axial sectional width of the concave portion along its own annular center. ### Claim 11 (depends on 1) The container with the embedded structure as claimed in any one of claim 1, further including a third plate; the third plate having a third end surface, the second plate having a fourth end surface located on another side of the second plate body and opposite to the second end surface, and the third end surface of the third plate being attached with the fourth end surface of the second plate, so that the second plate being sandwiched and pressed tightly between the first plate and the third plate. ### Claim 12 (depends on 11) The container with the embedded structure as claimed in claim 11, wherein the axial sectional width of the protrusion along its own annular center is not greater than the axial sectional width of the concave portion along its own annular center. ### Claim 13 (depends on 2) The container with the embedded structure as claimed in any one of claim 2, further including a third plate; the third plate having a third end surface, the second plate having a fourth end surface located on another side of the second plate body and opposite to the second end surface, and the third end surface of the third plate being attached with the fourth end surface of the second plate, so that the second plate being sandwiched and pressed tightly between the first plate and the third plate. ### Claim 14 (depends on 13) The container with the embedded structure as claimed in claim 13, wherein the axial sectional width of the protrusion along its own annular center is not greater than the axial sectional width of the concave portion along its own annular center. ### Claim 15 (depends on 3) The container with the embedded structure as claimed in any one of claim 3, further including a third plate; the third plate having a third end surface, the second plate having a fourth end surface located on another side of the second plate body and opposite to the second end surface, and the third end surface of the third plate being attached with the fourth end surface of the second plate, so that the second plate being sandwiched and pressed tightly between the first plate and the third plate. ### Claim 16 (depends on 15) The container with the embedded structure as claimed in claim 15, wherein the axial sectional width of the protrusion along its own annular center is not greater than the axial sectional width of the concave portion along its own annular center. ### Claim 17 (depends on 4) The container with the embedded structure as claimed in any one of claim 4, further including a third plate; the third plate having a third end surface, the second plate having a fourth end surface located on another side of the second plate body and opposite to the second end surface, and the third end surface of the third plate being attached with the fourth end surface of the second plate, so that the second plate being sandwiched and pressed tightly between the first plate and the third plate. ### Claim 18 (depends on 17) The container with the embedded structure as claimed in claim 17, wherein the axial sectional width of the protrusion along its own annular center is not greater than the axial sectional width of the concave portion along its own annular center. ### Claim 19 (depends on 5) The container with the embedded structure as claimed in any one of claim 5, further including a third plate; the third plate having a third end surface, the second plate having a fourth end surface located on another side of the second plate body and opposite to the second end surface, and the third end surface of the third plate being attached with the fourth end surface of the second plate, so that the second plate being sandwiched and pressed tightly between the first plate and the third plate. ### Claim 20 (depends on 19) The container with the embedded structure as claimed in claim 19, wherein the axial sectional width of the protrusion along its own annular center is not greater than the axial sectional width of the concave portion along its own annular center. ### Claim 21 (depends on 6) The container with the embedded structure as claimed in any one of claim 6, further including a third plate; the third plate having a third end surface, the second plate having a fourth end surface located on another side of the second plate body and opposite to the second end surface, and the third end surface of the third plate being attached with the fourth end surface of the second plate, so that the second plate being sandwiched and pressed tightly between the first plate and the third plate. ### Claim 22 (depends on 21) The container with the embedded structure as claimed in claim 21, wherein the axial sectional width of the protrusion along its own annular center is not greater than the axial sectional width of the concave portion along its own annular center. ### Claim 23 A container with an embedded structure comprising: a first plate with a first plate body, a first end surface located on one side of the first plate body; and a second plate with a second plate body, a second end surface opposite to the first end surface and located on one side of the second plate body, a sleeve portion annularly protruded on the second end surface to sleeve on the first plate to achieve a tightly connected state between the first plate and the sleeve portion in order to form an accommodating space between the first end surface, the second end surface, and the sleeve portion for a fluid to flow in the accommodating space, and the accommodating space having an accommodating height between the first end surface and the second end surface. ### Claim 24 (depends on 23) The container with the embedded structure as claimed in claim 23, wherein the second plate is made of flexible material. ### Claim 25 (depends on 24) The container with the embedded structure as claimed in claim 24, wherein the first plate further includes a protruding portion protruding on the first end surface, an outer diameter of the protruding portion is greater than an inner diameter of the sleeve portion, when an inner side surface of the sleeve portion is sleeved on a peripheral side of the protruding portion, the second plate is deformed to change the inner diameter of the sleeve portion. ### Claim 26 (depends on 25) The container with the embedded structure as claimed in claim 25, wherein a height of the protruding portion protruding from the first end surface is smaller than a height of the sleeve portion protruding from the second end surface. ### Claim 27 (depends on 25) The container with the embedded structure as claimed in claim 25, wherein the first plate further includes a groove located on the protruding portion, a predetermined groove depth is recessed from the first end surface toward the inner direction of the first plate body, and a groove depth of the groove constitutes the accommodating height. ### Claim 28 (depends on 25) The container with the embedded structure as claimed in claim 25, wherein the sleeve portion further includes a first sleeve body sleeved on the peripheral side of the protruding portion, and a second sleeve body provided on one side of the first sleeve body, a height of the first sleeve body protruding outside of the second end surface is greater than a height of the second sleeve body protruding outside of the second end surface, and a stopping distance between a free end of the first sleeve body and a free end of the second sleeve body is smaller than a height of the protruding portion, so that the free end of the second sleeve body abuts on the protruding portion, and a height of the second sleeve body constitutes the accommodating height. ### Claim 29 (depends on 25) The container with the embedded structure as claimed in claim 25, wherein the first plate further includes a groove located on the protruding portion, a predetermined groove depth is recessed from the first end surface toward the inner direction of the first plate body; the sleeve portion further includes a first sleeve body sleeved on the peripheral side of the protruding portion, and a second sleeve body provided on one side of the first sleeve body, a height of the first sleeve body protruding outside of the second end surface is greater than a height of the second sleeve body protruding outside of the second end surface, and a stopping distance between the free end of the first sleeve body and the free end of the second sleeve body is smaller than a height of the protruding portion, so that the free end of the second sleeve body abuts on the protruding portion, and a sum of the groove depth of the groove and the height of the second sleeve body constitutes the accommodating height.