# US 2021/0330516 A1 · Apparatus, system, and method for detecting a target substance > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0330516 A1 - Document ID: US20210330516A1 - Status: Published application - Kind: A1 - Application number: 16/320,294 - Filed: 2017-01-27 - Published: 2021-10-28 - Earliest claimed: 2015-10-06 - Assignees: Undercover Colors, Inc. - Applicants: Undercover Colors, Inc. - Inventors: Nicolas Letourneau; Aly Khalifa; Michael Gorczynski; Catherina Gomes; Ronald Smith; Sarah Paluskiewicz; Stephen Gray; Tyler Confrey-Maloney - Web page: https://patentgrep.com/patent/US20210330516A1 ## CPC - A61F 13/42: with wetness indicator or alarm - A61B 5/6826: {Finger} - A61F 13/00055: {Saturation indicators} - G01N 21/78: producing a change of colour - G01N 33/5306: {Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding} - G01N 33/54388: {based on lateral flow} - A61B 5/14517: {for sweat} - G01N 2021/757: {using immobilised reagents} - A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Described herein are apparatus, methods, and systems for detecting the presence of a targeted substance. The apparatus comprises a detection layer comprising an indicator that is configured to display a signal upon the detection of an interaction with the targeted substance. In some examples, the apparatus can include a top layer coupled to a top surface of the detection layer and a bottom layer coupled to a bottom surface of the detection layer. ## Claims ### Claim at position 1 1-18. (canceled) ### Claim 19 An apparatus for detecting the presence of a targeted substance in a liquid, the apparatus comprising: a bottom layer comprising a channel; a detection layer for receiving a liquid, wherein the detection layer is disposed in the channel, and wherein the detection layer is capable of displaying a signal that a targeted substance is present or absent; and a top layer disposed over the detection layer and coupled to the detection layer or to the bottom layer, wherein the top layer comprises at least one opening over a portion of the detection layer, and wherein the apparatus comprises a boundary that substantially prevents liquid entrainment in the apparatus. ### Claim 20 (depends on 19) The apparatus of claim 19, wherein the at least one opening is an entry port through which a fluid external to the apparatus can enter the apparatus and contact the detection layer. ### Claim 21 (depends on 19) The apparatus of claim 19, further comprising: a removable layer disposed over at least a portion of the top layer configured such that upon removal of the removable layer, at least a portion of the detection layer is exposed to an external environment. ### Claim 22 (depends on 19) The apparatus of claim 19, further comprising an opening in the apparatus to provide a vent for a gas within the apparatus. ### Claim 23 (depends on 19) The apparatus of claim 19, wherein the detection layer comprises a lateral flow assay. ### Claim 24 (depends on 19) The apparatus of claim 19, wherein the detection layer comprises a chromatographic membrane pad, a sample pad, a conjugate pad, and an absorbent pad, wherein the sample pad is configured to transfer the liquid to the conjugate pad; wherein the conjugate pad is configured to transfer the liquid to the chromatographic membrane pad; wherein the chromatographic membrane pad comprises a marker, overlaps a portion of the sample pad, and is configured to transfer the liquid to the absorbent pad; wherein the absorbent pad overlaps a portion of the chromatographic membrane pad, and is configured to draw the liquid from the chromatographic membrane pad; wherein the marker is configured to display a signal upon a detection of the targeted sub stance; wherein the signal is a visual indication that the target substance is present or absent. ### Claim 25 (depends on 24) The apparatus of claim 24, wherein the marker comprises carboxyfluorescein, 2,7-dichlorofluorescein, Eosin B, Eosin Y, erythrosine, fluorescein, fluorescein amidite, fluorescein isocyanate, gold nanoparticles, particles, aptamers, antibodies, merbromin, phloxine B, Rose Bengal, derivatives and salts thereof, or combinations thereof. ### Claim 26 (depends on 24) The apparatus of claim 24, wherein the signal comprises any one of: an appearance of a colored dot or region, an absence of any appearance of a colored region, a completion of a pattern, a completion of a line, a completion of a logo, a completion of a symbol, a printing of a word, checkmark, emoticon, symbol, fluorescence, vibration, or sound. ### Claim 27 (depends on 24) The apparatus of claim 24, wherein the signal is made by any one of: electrochemical detection, polymerization or de-polymerization in the presence of an analyte, endothermic reaction, exothermic reaction initiation, hydrogel formation, or electronic device-aided quantitation. ### Claim 28 (depends on 19) The apparatus of claim 19, wherein the target substance comprises any one of: amine-containing compound, benzodiazepine, narcotic, alcohol, date rape drug, pesticide, steroid, steroid metabolite, bacteria, pathogen, fungus, poison, toxin, explosive, explosive precursor material, metal, protein, and sugars. ### Claim 29 (depends on 19) The apparatus of claim 19, wherein the liquid comprises any one of: beer, cider, energy drink, flavored drink, fruit drink, liquor, alcoholic beverage, milk, milk-containing beverage, soda, sports drink, vegetable drink, water, wine, blood, non-potable water, organic solvent, potable water, serum, treated waste water, untreated waste water, urine, sweat, vomit, and combinations thereof ### Claim 30 (depends on 19) The apparatus of claim 19, wherein the bottom layer comprises a polymeric material comprising at least one of acrylonitrile butadiene styrene, acrylonitrile butadiene styrene and polycarbonate alloy, acetal polyoxymethylene, liquid crystal polymer, nylon 6-polyamide, nylon 6/6-polyamide, nylon 1 1-polyamide, polybutylene terepthalate polyester, polycarbonate, polyetherimid, polyethylene, low density polyethylene, high density polyethylene, polyethylene terepthalate polyester, polypropylene, polyphthalamide, polyphenylene sulfide, polystyrene crystal, high impact polystyrene, polysulfone, polyvinylchloride, polyvinylidene fluoride, styrene acrylonitrile, thermoplastic elastomer, thermoplastic polyurethane elastomer, cyclic olefin copolymer, or styrene butadiene copolymer. ### Claim 31 (depends on 19) The apparatus of claim 19, wherein the apparatus is fully submersible in a liquid with no effect on the detection layer. ### Claim 32 (depends on 19) The apparatus of claim 19, wherein the apparatus can be positioned on, within, or below one or more implements. ### Claim 33 (depends on 32) The apparatus of claim 32, wherein the one or more implements comprise at least one of a cup, a drinking straw, a bar coaster, a drink stirrer, a drink ornament, a charm, a sticker, and a lanyard. ### Claim 34 (depends on 19) A method of using an apparatus to detect the presence of a targeted substance in a liquid medium, said method comprising: providing the apparatus of claim 19; contacting a portion of the apparatus to the liquid; and observing an indication to determine presence or absence of the targeted substance. ### Claim 35 (depends on 34) The method of claim 34, wherein the targeted substance comprises any one of: amine-containing compounds, benzodiazepines, narcotics, alcohol, date rape drugs, pesticides, steroids, steroid metabolites, bacteria, pathogens, fungi, poisons, toxins, chemical warfare agents, environmental poisons, explosives, explosive precursor materials, small molecule mixtures, metals, volatile organics, allergens, proteins, and sugars. ### Claim 36 (depends on 34) The method of claim 34, wherein the liquid comprises any one of: beer, cider, energy drink, flavored drink, fruit drink, liquor, alcoholic beverage, milk, milk-containing beverage, soda, sports drink, vegetable drink, water, wine, blood, non-potable water, organic solvent, potable water, serum, treated waste water, untreated waste water, urine, vomit, and combinations thereof. ### Claim 37 A method of making an apparatus for detecting the presence of a targeted substance in a liquid medium comprising: providing a detection layer configured to detect the presence or absence of a targeted sub stance; coupling the detection layer to a bottom layer; and coupling a top layer over the detection layer, wherein the apparatus is capable of being positioned on, within, or below an implement. ### Claim 38 (depends on 37) The method of claim 37, further comprising coupling a removable layer to the apparatus, wherein the removable layer covers at least a portion of the top layer.