# US 2021/0329944 A1 · Bacillus subtilis for animal feed > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0329944 A1 - Document ID: US20210329944A1 - Status: Published application - Kind: A1 - Application number: 16/471,298 - Filed: 2019-01-18 - Published: 2021-10-28 - Earliest claimed: 2018-01-19 - Assignees: Chr. Hansen A/S - Applicants: Chr. Hansen A/S - Inventors: Dorthe SANDVANG; Ole Knudsmark SJOEHOLM; Line SKJOET-RASMUSSEN; Jette Mundus NIELSEN - Web page: https://patentgrep.com/patent/US20210329944A1 ## CPC - A23K 50/40: for carnivorous animals, e.g. cats or dogs - A01N 63/22: Bacillus - A23K 10/18: of live microorganisms - C12N 1/20: Bacteria; Culture media therefor - C12N 1/205: Bacterial isolates - C12R 2001/125: Bacillus subtilis {; Hay bacillus; Grass bacillus} - A23K: FODDER - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present invention provides a Bacillus subtilis strain deposited as DSM 32685 and mutant strains thereof which are susceptible to relevant antibiotics, have inhibitory activity against Staphylococcus, Streptococcus, E. coli and Salmonella enterica and have the ability to facilitate degradation of non-starch polysaccharides and thereby increase the amount of available oligosaccharides (sugar) in animal feed. The invention further relates to a composition comprising at least one Bacillus subtilis strain of the invention and optionally further bacteria, and/or one or more types of yeast. The composition may be a probiotic, Direct Fed Microbial (DFM), a premix, an animal feed additive, or an animal feed such as complete feed, e.g. a canine complete feed. The composition can be used in the prevention, reduction or control of a bacterial colonization or infection or for increasing digestibility of an animal feed, in particular a feed for a monogastric animal, such as feed for a companion animal, e.g. a domestic dog or cat. ## Claims ### Claim 1 A Bacillus subtilis strain selected from the group consisting of a) the strain deposited as DSM 32685 and b) a mutant strain of DSM 32685 which (i) has susceptibility to ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol tested after the CLSI guidelines (M07-A8 and M45-A2); (ii) exhibits an inhibition zone of at least 1 mm for Staphylococcus pseudintermedius, E. coli and Salmonella enterica; and (iii) reduces the growth rate of Streptococcus canis by at least one Log 10 growth in 24 hours. ### Claim 2 (depends on 1) A composition comprising at least one Bacillus subtilis strain of claim 1. ### Claim 3 (depends on 2) The composition according to claim 2 which comprises the Bacillus subtilis strain deposited as DSM 32685. ### Claim 4 (depends on 2, 3) The composition of claim 2 or 3 which comprises a bacterium from one or more of the following genera: Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium and Megasphaera or any combination thereof. ### Claim 5 (depends on 2, 3, 4) The composition of any one of claims 2 to 4 which further comprises one or more types of yeast. ### Claim 6 (depends on 2, 3, 4, 5) The composition of any one of claims 2 to 5, wherein the Bacillus strain or strains are spores. ### Claim 7 (depends on 2, 3, 4, 5, 6) The composition according to any one of claims 2 to 6 which is a probiotic, Direct Fed Microbial (DFM), a premix, an animal feed additive, or an animal feed such as complete feed. ### Claim 8 (depends on 2, 3, 4, 5, 6, 7) A composition according to any one of claims 2 to 7 for use in the prevention, reduction or control of a bacterial colonization or infection. ### Claim 9 (depends on 8) The composition according to claim 8 for use in the prevention, reduction or control of a bacterial colonization or infection by Staphylococcus and/or Streptococcus and/or E. coli and/or Salmonella enterica. ### Claim 10 (depends on 1, 2, 3, 4, 5, 6, 7) A method for the prevention, reduction or control of a bacterial colonization or infection, the method comprising administering an effective amount of a strain according to claim 1 or a composition according to any one of claims 2 to 7 to an animal in need thereof. ### Claim 11 (depends on 10) The method according to claim 10 wherein colonization or infection by Staphylococcus and/or Streptococcus and/or E. coli and/or Salmonella enterica is prevented or controlled. ### Claim 12 (depends on 1, 2, 3, 4, 5, 6, 7) A method for feeding an animal comprising administering a strain according to claim 1 or a composition according to any one of claims 2 to 7 to an animal. ### Claim 13 (depends on 12) The method according to claim 12, wherein the animal is a monogastric animal, such as a companion animal, e.g. a dog or a cat. ### Claim 14 (depends on 12, 13) A method according to claim 12 or 13 of increasing digestibility of an animal feed. ### Claim 15 (depends on 12, 13, 14) A method according to any one of claims 12 to 14 of improving one or more animal performance parameters selected from the group consisting of i) increased weight gain (WG), ii) lower feed conversion ratio (FCR), iii) increase the digestibility of the feed, iv) lower enteritis frequency, iv) increased European Production Efficacy Factor (EPEF), and v) lower mortality.