# US 2021/0329932 A1 · Rosemary/phospholipase compositions and methods of preserving muscle tissue

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- Number: US 2021/0329932 A1
- Document ID: US20210329932A1
- Status: Published application
- Kind: A1
- Application number: 17/238,631
- Filed: 2021-04-23
- Published: 2021-10-28
- Earliest claimed: 2016-04-21
- Assignees: Wisconsin Alumni Research Foundation
- Applicants: Wisconsin Alumni Research Foundation
- Inventors: Mark P. RICHARDS; Jie YIN; Wenjing ZHANG
- Web page: https://patentgrep.com/patent/US20210329932A1

## CPC

- A23B 4/22: Microorganisms; Enzymes; {Antibiotics}
- A23B 2/733: Compounds of undetermined constitution obtained from animals or plants
- A23B 2/783: Microorganisms; Enzymes
- A23B 4/18: in the form of liquids or solids (apparatus therefor A23B4/26, A23B4/32)
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23V 2250/21: Plant extracts
- A23V 2250/54: Proteins
- A23B: PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The disclosure provides for compositions and methods for the preservation of meat tissues, including fish, beef, poultry and pork, and meat analogs containing added heme protein, using very low amounts of phospholipase A2 (PLA2) enzymes in a combination with rosemary.

## Claims

### Claim 1

A method of improving storage life of comminuted muscle tissue or meat analog containing added heme protein, comprising
contacting the tissue with about 50 U/kg to about 500 U/kg phospholipase A2 enzyme (PLA2) and rosemary extract at about 150 ppm to about 525 ppm, wherein the muscle tissue is selected from the group consisting of avian tissue and mammalian tissue.

### Claim at position 2

2-5. (canceled)

### Claim 6 (depends on 1)

The method of claim 1, wherein the rosemary extract is contacted at a concentration of no more than about 250 ppm.

### Claim 7 (depends on 1)

The method of claim 1, wherein the rosemary extract is contacted at a concentration of about 175 ppm to about 225 ppm.

### Claim at position 5

8-10. (canceled)

### Claim 11 (depends on 1)

The method of claim 1, wherein the rosemary extract is contacted at a concentration of about 200 ppm.

### Claim 12 (depends on 1)

The method of claim 1, wherein the PLA2 enzyme is contacted at a concentration of about 50 U/kg.

### Claim at position 8

13-15. (canceled)

### Claim 16 (depends on 1)

The method of claim 1, wherein the PLA2 enzyme is contacted at a concentration of no more than about 525 U/kg.

### Claim 17 (depends on 1)

The method of claim 1, wherein the PLA2 enzyme is contacted at a concentration of about 63 U/kg to about 450 U/kg.

### Claim at position 11

18-20. (canceled)

### Claim 21 (depends on 1)

The method of claim 1, wherein the PLA2 enzyme is contacted at a concentration of about 250 U/kg.

### Claim 22 (depends on 1)

The method of claim 1, wherein the muscle tissue is avian tissue.

### Claim 23

(canceled)

### Claim 24 (depends on 1)

The method of claim 1, wherein the tissue is mammalian tissue.

### Claim 25 (depends on 1)

The method of claim 1, wherein the tissue is red meat.

### Claim at position 17

26-28. (canceled)

### Claim 29 (depends on 1)

The method of claim 1, wherein the muscle tissue is cooked or cured muscle tissue.

### Claim 30 (depends on 1)

The method of claim 1, wherein the muscle tissue is uncooked and uncured.

### Claim 31 (depends on 1)

The method of claim 1, wherein the meat analog containing added heme protein is treated with bacterial PLA2.

### Claim 32 (depends on 1)

The method of claim 1, further comprising freezing the muscle tissue.

### Claim 33 (depends on 1)

The method of claim 1, wherein the muscle tissue or meat analog is frozen at 0 to 6° C.

### Claim 34 (depends on 1)

The method of claim 1, wherein the muscle tissue or meat analog is treated substantially in the absence of exogenous calcium.

### Claim 35 (depends on 1)

The method of claim 1, wherein the muscle tissue contains hemoglobin at levels that are 80% of fresh un-stored tissue for 2, 3, 4, 5, 6, 7, 8, 9 or 10 days following treatment with the PLA2 enzyme and rosemary extract.

### Claim at position 25

36-37. (canceled)

### Claim 38

A storage-stable muscle tissue or meat analog containing added heme protein comprising about 50 U/kg to about 525 U/kg phospholipase A2 enzyme (PLA2) and rosemary extract at about 150 ppm to about 250 ppm, wherein the muscle tissue is selected from the group consisting of comminuted avian tissue and comminuted mammalian tissue.