# US 2021/0329927 A1 · Microalgae based compositions and methods for application to plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0329927 A1 - Document ID: US20210329927A1 - Status: Published application - Kind: A1 - Application number: 17/239,434 - Filed: 2021-04-23 - Published: 2021-10-28 - Earliest claimed: 2015-09-11 - Assignees: Heliae Development, LLC - Applicants: Heliae Development LLC - Inventors: Sandip Shinde; Stephen Ventre; Manikandadas Mathilakathu Madathil; Laura Carney; Jerald Wheeler - Web page: https://patentgrep.com/patent/US20210329927A1 ## CPC - A01N 65/03: Algae - A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS ## Abstract Microalgae based compositions and methods of improving emergence and yield of plants by administering an effective amount of a microalgae based liquid composition in combination with other active ingredients including extracts from macroalgae, extracts from microalgae, minerals, humate derivatives, primary nutrients, micronutrients, chelating agents, and anti-biotics are disclosed. A method of applying a microalgae based composition to soil to increase the cation exchange capacity of the soil is also disclosed. ## Claims ### Claim 1 A method of increasing root nodule formation in a plant, the method comprising administering to a plant, seedling, or seed a composition comprising 0.001-30% by volume of microalgae cells in combination with free living nitrogen fixing bacteria, cytokinin producing bacteria, or a combination of both. ### Claim 2 (depends on 1) The method of claim 1, wherein the microalgae cells are Chlorella cells. ### Claim 3 (depends on 2) The method of claim 2, wherein the Chlorella cells are whole cells, lysed cells, dried cells, or cells that have been subjected to an extraction process. ### Claim 4 (depends on 2) The method of claim 2, wherein the Chlorella cells are pasteurized. ### Claim 5 (depends on 1) The method of claim 1, wherein the cytokinin producing bacteria comprise Methylotrophs, Methylobacterium sp., Xanthobacter sp., Paracoccus sp., Rhizobium sp., Sinorhizobium sp., or Methyloversatilis. ### Claim 6 (depends on 5) The method of claim 5, wherein the cytokinin producing bacteria comprise Rhizobium sp. ### Claim 7 (depends on 5) The method of claim 5, wherein the cytokinin producing bacteria comprise Sinorhizobium sp. ### Claim 8 (depends on 1) The method of claim 1, wherein the plant belongs to the Fabaceae family and is selected from the group consisting of soybeans, beans, green beans, peas, chickpeas, alfalfa, peanuts, sweet peas, carob, and liquorice. ### Claim 9 (depends on 8) The method of claim 8, wherein the plant is soybeans. ### Claim 10 (depends on 1) The method of claim 1, wherein the administrating is selected from: treating a seed with the composition prior to planting; administering the composition to a solid growth medium prior to or after the planting of a seed, seedling, or plant; and administering the composition to the foliage of a seedling or plant. ### Claim 11 (depends on 10) The method of claim 10, wherein the solid growth medium comprises at least one from the group consisting of: soil, potting mix, compost, or inert hydroponic material. ### Claim 12 A method of increasing root nodule formation in a plant, the method comprising administering to a plant, seedling, or seed a composition comprising 0.001-30% by volume of Chlorella cells in combination with cytokinin producing bacteria selected from the group consisting of Methylotrophs, Methylobacterium sp., Xanthobacter sp., Paracoccus sp., Rhizobium sp., Sinorhizobium sp., and Methyloversatilis. ### Claim 13 (depends on 12) The method of claim 12, wherein the Chlorella cells are whole cells, lysed cells, dried cells, or cells that have been subjected to an extraction process. ### Claim 14 (depends on 12) The method of claim 12, wherein the Chlorella cells are pasteurized. ### Claim 15 (depends on 12) The method of claim 12, wherein the composition further comprises bacteria that produce a siderophore or indole acetic acid (IAA). ### Claim 16 (depends on 12) The method of claim 12, wherein the plant belongs to the Fabaceae family and is selected from the group consisting of soybeans, beans, green beans, peas, chickpeas, alfalfa, peanuts, sweet peas, carob, and liquorice. ### Claim 17 (depends on 12) The method of claim 12, wherein the administrating is selected from: treating a seed with the composition prior to planting; administering the composition to a solid growth medium prior to or after the planting of a seed, seedling, or plant; and administering the composition to the foliage of a seedling or plant. ### Claim 18 (depends on 17) The method of claim 17, wherein the solid growth medium comprises at least one from the group consisting of: soil, potting mix, compost, or inert hydroponic material. ### Claim 19 A method of increasing root nodule formation in a plant, the method comprising administering to a plant, seedling, or seed a composition comprising 0.001-30% by volume of Chlorella cells in combination with Sinorhizobium sp. ### Claim 20 (depends on 19) The method of claim 19, wherein the plant belongs to the Fabaceae family and is selected from the group consisting of soybeans, beans, green beans, peas, chickpeas, alfalfa, peanuts, sweet peas, carob, and liquorice.