# US 2021/0329893 A1 · Method for establishing an oral ulcer animal model with yin-deficiency and fire-excess syndrome based on sleep deprivation

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- Number: US 2021/0329893 A1
- Document ID: US20210329893A1
- Status: Published application
- Kind: A1
- Application number: 16/615,009
- Filed: 2019-04-29
- Published: 2021-10-28
- Earliest claimed: 2018-08-29
- Assignees: Sun Yat-Sen University
- Applicants: Sun Yat-Sen University
- Inventors: Hongliang YAO; Pan SHEN; Peibo LI; Weiwei SU
- Web page: https://patentgrep.com/patent/US20210329893A1

## CPC

- A01K 67/00: Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K 67/027: New or modified breeds of vertebrates
- A01K 2207/20: Animals treated with compounds which are neither proteins nor nucleic acids
- A01K 2207/35: Animals modified by environmental factors, e.g. temperature, O2
- A01K 2227/105: Murine
- A01K 2267/03: Animal model, e.g. for test or diseases
- A01K 2267/0368: Animal model for inflammation
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS

## Abstract

The present disclosure relates to a method for establishing an oral ulcer animal model with Yin-deficiency and fire-excess syndrome based on sleep deprivation, comprising the following steps of: burning buccal mucosa of a rat with phenol to cause a white injury, and obtaining the oral ulcer animal model after 24-48 h; placing the animal model in a sleep deprivation device for 2-7 days; wherein the device is composed of a mouse box in which a plurality of platforms with a diameter of 4-8 cm are arranged at an interval of 10-20 cm from each other; and water is filled among the platforms, and the platforms are about 0.1-2 cm higher than a water surface. The present disclosure overcomes the defects of single evaluation index, short model duration, and unconformity of etiology and clinic existed in the prior art.

## Claims

### Claim 1

A method for establishing an oral ulcer animal model with Yin-deficiency and fire-excess syndrome based on sleep deprivation, comprising the following steps of:
preparing an oral ulcer animal model: contacting phenol with buccal mucosa of a rat to form a white corrosion spot, and obtaining the oral ulcer animal model after 24 h to 48 h; and
establishing a model with Yin-deficiency and fire-excess syndrome based on sleep deprivation: placing the oral ulcer animal model in a sleep deprivation device for 2 days to 7 days, wherein the device is composed of a mouse box in which a plurality of platforms with a diameter of 4 cm to 8 cm are arranged at an interval of 10 cm to 20 cm from each other; and water is filled among the platforms, and the platforms are about 0.1 cm to 2 cm higher than a water surface.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the platforms have a diameter of 6.3 cm.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the platforms have an interval of 15 cm from each other.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the platforms are 1 cm higher than the water surface.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the platforms have a height of 5 cm to 10 cm.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the water has a temperature of 20° C. to 25° C.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the rat is a SD rat with a weight of 150 g to 350 g.

### Claim 8 (depends on 1)

The method according to claim 1, wherein the oral ulcer animal model is placed in the sleep deprivation device for 3 days.