In vitro Transcription-Translation Metabolic Networks to Measure Quantity or Activity of Enzymes
AssigneeBrigham Young University
InventorsBradley C. BUNDY, J. Porter HUNT
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Abstract
Disclosed herein are biosensors including transcription, translation, and coupled transcription/translation systems. The biosensors may be made from cell lysates, purified enzymes, or a combination thereof. The biosensors may include an inhibitor, and may include a reporter. The biosensor may be supplied in a kit for testing for a disease or condition. Methods of using the biosensor are also disclosed.
Claims31
An in vitro system for detecting glutamine in a sample, the in vitro system comprising:
- at least one of a transcription system and a translation system;
- an inhibitor of glutamine biosynthesis;
- and
- a reporter of transcription and/or translation in the system;
- wherein the reporter provides a change in signal proportionate to rescue of transcription and/or translation by the sample relative to a level of transcription and/or translation in the system when the sample is absent.
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An in vitro system for measuring a biological activity in a sample, the in vitro system comprising:
- a coupled transcription-translation system;
- an inhibitor of the coupled transcription-translation system;
- and
- a reporter of the biological activity;
- wherein the reporter is inactive when the sample is absent, and wherein the reporter provides a signal proportionate to rescue of the biological activity by the sample.
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Dependencies are inferred from the claim text.