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US 2021/0324439 A1Published application
Methods for making high intensity sweeteners
AssigneeFirmenich SA
InventorsAndrew P. Patron, Chris Edano Noriega, Rama R. Manam, Justin Colquitt, Nathan Faber, Helge Zieler, Justin Stege
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Abstract
Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof.
Claims20
A method of producing Compound 1 having the structure of:
- the method comprising:
- contacting mogroside IIE with a first enzyme capable of catalyzing a production of Compound 1 from mogroside IIE;
- wherein the first enzyme is one or more of UDP glycosyltransferases, cyclomaltodextrin glucanotransferases (CGTases), glycotransferases, dextransucrases, cellulases, β-glucosidases, amylases, transglucosidases, pectinases, and dextranases;
- wherein the CGTases comprise an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs:
- 3, 78-88, 90-101, 148, and 154;
- wherein the dextransucrases comprise an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs:
- 106-110, 156, and 896;
- or
- wherein the enzyme having dextransucrase activity is encoded by a gene having at least 90% sequence identity to any one of SEQ ID NOs:
- 157 and 895.
7 more elements
Dependencies are inferred from the claim text.