Nucleotide sequence encoding 9-lipoxygenase and recombinant constructs comprising the same

A process for synthesis of recombinant 9-lipoxygenase comprising:

• a) synthesizing polynucleotide having the nucleotide sequence as set forth in SEQ ID NO:
• 1 with primers having sequence selected from the group consisting of SEQ ID NO:
• 4, SEQ ID NO:5, SEQ ID NO:
• 6, and SEQ ID NO:
• 7;
• b) cloning the polynucleotide having the nucleotide sequence as set forth in SEQ ID NO:
• 1 obtained in step (a) into a plasmid expression vector using the expression vector specific primers to obtain a recombinant plasmid expression vector;
• c) transforming the recombinant plasmid expression vector obtained in step (b) into a host cell to obtain a transformed host cell;
• d) culturing the transformed host cell obtained in step (c) at a temperature ranging from 12° C. to 25° C. in a culture medium;
• e) separating the recombinant host cells from the culture medium;
• and
• f) performing lysis and sonication of recombinant host cells to isolate the recombinant 9-lipoxygenase.