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US 2021/0317493 A1Published application
Fermentative production of oligosaccharides by total fermentation utilizing a mixed feedstock
AssigneeJennewein Biotechnologie GmbH
InventorsStefan JENNEWEIN, Dirk WARTENBERG, Katja PARSCHAT
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Abstract
Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-β1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.
Claims20
A genetically engineered microbial cell for the production of lactose or an oligosaccharide of interest which comprises a galactose-β1,4-glucose moiety at its reducing end, wherein said microbial cell possesses:
- at least one glucose transporter for translocating glucose from the culture medium into the microbial cell's cytoplasm;
- an UDP-galactose biosynthesis pathway;
- and
- at least one β-1,4-galactosyltransferase being able to galactosylate free glucose to intracellularly produce lactose.
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Dependencies are inferred from the claim text.