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US 2021/0317433 A9Published application
Bio-Manufacturing Process
AssigneeEcovative Design LLC
InventorDamen Schaak
Life of this patent
Estimating the term…
Abstract
The process of making a biocomposite material utilize a bacterial species and a fungal species in an agricultural feedstock composed of a substrate of non-nutrient discrete particles and a nutrient material wherein the bacterial species imparts mechanical properties to the biocomposite material and the fungal species binds the biocomposite material. Both bacterium and fungus can be genetically engineered to produce desired properties within the microbial communities.
Claims19
A process of making a biocomposite material comprising the steps of
- forming a substrate of non-nutrient discrete particles and a nutrient material;
- adding a filamentous fungus to said substrate;
- adding a Bacillus subtilis strain characterized in producing a bio-film with poly-gamma-glutamic acid (PGA) to said substrate;
- co-cultivating said fungus and said Bacillus subtilis strain in said substrate and allowing said fungus to digest said nutrient material in said substrate over a period sufficient to grow hyphae and to allow said hyphae to form a network of interconnected mycelia cells through and around said non-nutrient discrete particles thereby bonding said discrete particles together to form a self-supporting composite material.
1 more element
A biocomposite material comprising
- a substrate of non-nutrient discrete particles;
- a bio-film containing poly-gamma-glutamic acid (PGA) dispersed within said substrate;
- and
- a network of interconnected mycelia cells extending through and around said discrete particles and bonding said discrete particles together.
1 more element
A process of making a biocomposite material comprising the steps of
- forming a substrate of non-nutrient discrete particles and a nutrient material;
- adding a filamentous fungus to said substrate;
- adding a Bacillus subtilis strain characterized in producing melanin to said substrate;
- co-cultivating said fungus and said Bacillus subtilis strain in said substrate and allowing said fungus to digest said nutrient material in said substrate over a period sufficient to grow hyphae and to allow said hyphae to form a network of interconnected mycelia cells through and around said non-nutrient discrete particles thereby bonding said discrete particles together to form a self-supporting composite material.
1 more element
A biocomposite material comprising
- a substrate of non-nutrient discrete particles;
- an amount of melanin dispersed within said substrate;
- and
- a network of interconnected mycelia cells extending through and around said discrete particles and bonding said discrete particles together, said biocomposite material being characterized in being radiation hard.
1 more element
A process of making a biocomposite material comprising the steps of forming a substrate of non-nutrient discrete particles and a nutrient material;
- adding a selected fungus to said substrate;
- adding Streptomyces natalensis characterized in producing natamycin to said substrate;
- co-cultivating said fungus and said Streptomyces natalensis in said substrate and allowing said fungus to digest said nutrient material in said substrate over a period sufficient to grow hyphae and to allow said hyphae to form a network of interconnected mycelia cells through and around said non-nutrient discrete particles thereby bonding said discrete particles together to form a self-supporting composite material while allowing said Streptomyces natalensis to produce natamycin as a fungicide in said self-supporting composite material.
A biocomposite material comprising
- a substrate of non-nutrient discrete particles;
- an amount of natamycin dispersed within said substrate;
- and
- a network of interconnected mycelia cells extending through and around said discrete particles and bonding said discrete particles together, said biocomposite material being characterized in having fungicidal properties.
1 more element
A process of making a biocomposite material comprising the steps of
- forming a substrate of non-nutrient discrete particles and a nutrient material;
- adding a filamentous fungus to said substrate;
- adding a Bacillus subtilis strain characterized in producing an antifungal protein (AFP1) native to Streptomyces tendae to said substrate;
- co-cultivating said fungus and said Bacillus subtilis strain in said substrate and allowing said fungus to digest said nutrient material in said substrate over a period sufficient to grow hyphae and to allow said hyphae to form a network of interconnected mycelia cells through and around said non-nutrient discrete particles thereby bonding said discrete particles together to form a self-supporting composite material.
1 more element
A biocomposite material comprising
- a substrate of non-nutrient discrete particles;
- an amount of an antifungal protein (AFP1) native to Streptomyces tendae dispersed within said substrate;
- and
- a network of interconnected mycelia cells extending through and around said discrete particles and bonding said discrete particles together, said biocomposite material being characterized in having fungicidal properties.
1 more element
A process comprising the steps of
- obtaining a feedstock including non-nutrient discrete particles, a nutrient material and at least one native microbial species;
- isolating said at least one native microbial species from said feedstock;
- subjecting said isolated native microbial species to genetic processing to transform said native microbial species into a genetically engineered microbial species having predetermined characteristics;
- and
- thereafter returning said genetically engineered microbial species into said feedstock.
2 more elements
A process of making a biocomposite material comprising the steps of
- forming a substrate of non-nutrient discrete particles and a nutrient material;
- adding a filamentous fungus genetically engineered to have predetermined characteristics to said substrate;
- adding a bacterial species to said substrate;
- and
- co-cultivating said fungus and said bacterial species in said substrate and allowing said fungus to digest said nutrient material in said substrate over a period sufficient to grow hyphae and to allow said hyphae to form a network of interconnected mycelia cells through and around said non-nutrient discrete particles thereby bonding said discrete particles together to form a self-supporting composite material.
2 more elements
Dependencies are inferred from the claim text.