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US 2021/0317419 A1Published application
Microorganism expressing active d-proline reductase and method of producing active d-proline reductase
AssigneeCj Cheiljedang Corporation
InventorsJinseung Park, Bo Seong Park, Young Lyeol Yang, In Seok Oh, Nahum Lee, Jun Ok Moon
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Abstract
The present disclosure relates to a microorganism expressing active D-proline reductase.
Claims24
A microorganism expressing active D-proline reductase, wherein activities of PrdA protein, PrdB protein, and PrdH protein are enhanced.
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A PrdH protein having activity to convert inactive D-proline reductase into active D-proline reductase while being co-expressed with PrdA protein and PrdB protein.
A method of producing a microorganism expressing active D-proline reductase, the method comprising:
- transforming a host cell with an expression cassette comprising prdA gene or prdA and prdH genes;
- and
- transforming the host cell with an expression cassette comprising prdB gene or prdB and prdH genes.
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Dependencies are inferred from the claim text.