Improved recovery of nitrate reductase activity
AssigneeChr. Hansen A/S
InventorsTina Malling THORSEN, George Nabin BAROI, Robin TAPONEN, Jakob SOELTOFT-JENSEN, Birgitte YDE
Life of this patent
Estimating the term…
Abstract
The present invention is related to the field of reddening of food products. In particular the present invention relates to the preservation or optimization of nitrate reductase activity of frozen and/or dried lactic acid bacteria cultures or Micrococcaceae cultures (particularly cultures comprising one or more species of Staphylococcus having nitrate reductase activity).
Claims92
A concentrated culture comprising a formulation of citric acid or a salt thereof and a bacterium having nitrate reductase activity selected from the group consisting of lactic acid bacteria and Micrococcaceae bacteria (e.g. a Staphylococcus specie).
The concentrated culture according to any one of the preceding claims, wherein the lactic acid bacteria is a specie of the Staphylococcus genus having nitrate reductase activity.
The concentrated culture according to any one of the preceding claims, wherein the lactic acid bacteria is a Staphylococcus specie is selected from the group consisting of Staphylococcus xylosus, Staphylococcus carnosus and Staphylococcus vitulinus.
The concentrated culture according to any one of the preceding claims, wherein the Staphylococcus specie is selected from the group consisting of Staphylococcus xylosus and Staphylococcus vitulinus.
The concentrated culture according to any one of the preceding claims, wherein the Staphylococcus specie is selected from the group consisting of Staphylococcus carnosus CHCC4055 deposited at Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under the accession No. DSM 32779, Staphylococcus vitulinus specie CHCC10896 deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under the accession no. DSM 25789, and the Staphylococcus vitulinus specie CHCC11576 deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under the accession no. DSM 27311 and mutants derived thereof.
The concentrated culture according to any one of the preceding claims, wherein the Staphylococcus specie is Staphylococcus vitulinus specie CHCC10896 deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under the accession no. DSM 25789 and mutants derived thereof.
The concentrated culture according to any one of the preceding claims, wherein the Staphylococcus specie is Staphylococcus vitulinus specie CHCC11576 deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under the accession no. DSM 27311 and mutants derived thereof.
The concentrated culture according to any one of the preceding claims, wherein the Staphylococcus carnosus specie is Staphylococcus carnosus CHCC4055 deposited at Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under the accession No. DSM 32779 and mutants derived thereof.
The concentrated culture according to any one of the preceding claims, wherein the formulation comprises a citrate salt (e.g. trisodium citrate).
The concentrated culture according to any one of the preceding claims, further comprising a monosaccharide, a disaccharide, a trisaccharide and/or an oligosaccharide and/or a polysaccharide.
The concentrated culture according to any one of the preceding claims, wherein the disaccharide may be selected from the group consisting of sucrose, lactose, maltose and trehalose.
The concentrated culture according to any one of the preceding claims, wherein said concentrated culture comprises or consists of one or more species of lactic acid bacteria and/or Micrococcaceae, (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, and a disaccharide, preferably sucrose.
The concentrated culture according to any one of the preceding claims, further comprising sucrose.
The concentrated culture according to any one of the preceding claims, further comprising a polysaccharide (e.g. maltodextrin).
The concentrated culture according to any one of the preceding claims further comprising maltodextrin, such as maltodextrin DE 3 to 20, e.g. maltodextrin DE 12.
The concentrated culture according to any one of the preceding claims further comprising maltodextrin DE 12.
The concentrated culture according to any one of the preceding claims, wherein said concentrated culture comprises or consists of one or more species of a lactic acid bacteria and/or Micrococcaceae (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein said concentrated culture comprises or consists of one or more species of a lactic acid bacteria and/or Micrococcaceae (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, a disaccharide (e.g. sucrose), a polysaccharide (e.g. maltodextrin), and optionally water.
The concentrated culture according to any one of the preceding claims, wherein said concentrated culture consists of one or more species of a lactic acid bacteria and/or Micrococcaceae, (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, a disaccharide (e.g. sucrose), and a polysaccharide (e.g. maltodextrin), and optionally water.
The concentrated culture according to any one of the preceding claims, wherein said concentrated culture comprises or consists of one or more species of Staphylococcaceae (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, a disaccharide (e.g. sucrose), and a polysaccharide (e.g. maltodextrin), and optionally water.
The concentrated culture according to any one of the preceding claims, wherein said concentrated culture consists of one or more species of Staphylococcaceae (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, a disaccharide (e.g. sucrose), and a polysaccharide (e.g. maltodextrin).
The concentrated culture according to any one of the preceding claims further comprising a sugar alcohol or a derivative thereof.
The concentrated culture according to any one of the preceding claims further comprising inositol or a derivative thereof (e.g. form of inositol phosphate).
The concentrated culture according to any one of the preceding claims further comprising inositol or a derivative thereof.
The concentrated culture according to any one of the preceding claims comprising or consisting of a lactic acid bacterium and/or Micrococcaceae bacteria (e.g. a Staphylococcus specie) having nitrate reductase activity, citric acid or a salt thereof, and inositol or a derivative thereof (e.g. a form of inositol phosphate).
The concentrated culture according to any one of the preceding claims comprising or consisting of a Staphylococcus specie having nitrate reductase activity, citric acid or a salt thereof, and inositol.
The concentrated culture according to any one of the preceding claims further comprising an amino acid, a peptide and/or a protein.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is dry.
The concentrated culture according to any one the preceding claims, wherein the concentrated culture is frozen, freeze-dried, vacuum-dried and/or spray-dried.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of a lactic acid bacteria and/or Micrococcaceae, (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, and a disaccharide, preferably sucrose.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of Staphylococcus, citric acid or a salt thereof, and a disaccharide, preferably sucrose.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of a lactic acid bacteria and/or Micrococcaceae (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the composition is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of Staphylococcus, citric acid or a salt thereof, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of a lactic acid bacteria and/or Micrococcaceae, (e.g. one or more species of Staphylococcus), citric acid or a salt thereof, a disaccharide, preferably sucrose, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of Staphylococcus, citric acid or a salt thereof, a disaccharide, preferably sucrose, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of Staphylococcus, citric acid or a salt thereof, a disaccharide, preferably sucrose, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a frozen, freeze-dried, vacuum-dried and/or spray-dried concentrated culture comprising one or more species of Staphylococcus selected from the group consisting of Staphylococcus xylosus, Staphylococcus carnosus and Staphylococcus vitulinus, citric acid or a salt thereof, a disaccharide, preferably sucrose, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a freeze-dried concentrated culture comprising one or more species of Staphylococcus selected from the group consisting of Staphylococcus vitulinus species or a Staphylococcus carnosus species, citric acid or a salt thereof, a disaccharide, preferably sucrose, and a polysaccharide, preferably maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a freeze-dried concentrated culture comprising or consisting of one or more species of Staphylococcus selected from the group consisting of Staphylococcus vitulinus species or a Staphylococcus carnosus species, citric acid or a salt thereof, sucrose, and maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is a freeze-dried concentrated culture comprising or consisting of one or more species of Staphylococcus selected from the group consisting of Staphylococcus vitulinus species or a Staphylococcus carnosus species, citric acid or a salt thereof, and inositol or a derivative thereof (e.g. inositol phosphate).
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is dry (e.g. freeze-dried) and comprises from about 0.1% w/w to about 30% w/w citric acid or a salt thereof (e.g. trisodium citrate).
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is dry (e.g. freeze-dried) and comprises from about 0.5% w/w to about 25% w/w citric acid or a salt thereof (e.g. trisodium citrate).
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is dry (e.g. freeze-dried) and comprises from about 1.0% w/w to about 20.0% w/w citric acid or a salt thereof (e.g. trisodium citrate).
The concentrated culture according to any one of the preceding claims, wherein the concentrated culture is dry (e.g. freeze-dried) and comprises from about 1.0% w/w to about 20.0% w/w citric acid or a salt thereof (e.g. trisodium citrate).
The concentrated culture according to any one of the preceding claims, wherein the culture is dry (e.g. freeze-dried) and comprises from about 1.0% w/w to about 10.0% w/w citric acid or a salt thereof (e.g. trisodium citrate), e.g. from about 2.0% w/w to about 8.0% w/w citric acid or a salt thereof.
The concentrated culture according to any one of the preceding claims, wherein the culture is dry (e.g. freeze-dried) and comprises from about 0.5% w/w to about 30.0% w/w sucrose, e.g. from about 1.0% w/w to about 8.0% w/w sucrose, or e.g. from about 2.0% w/w to about 5.0% w/w sucrose.
The concentrated culture according to any one of the preceding claims, wherein the culture is dry (e.g. freeze-dried) and comprises from about 0.5% w/w to about 30.0% w/w maltodextrin, e.g. from about 1.0% w/w to about 15.0% w/w maltodextrin, or e.g. from about 2.0% w/w to about 12.0% w/w maltodextrin.
The concentrated culture according to any one of the preceding claims, wherein the culture is dried (e.g. freeze-dried) and comprises from about 0.5% w/w to about 20.0% w/w sugar alcohol (e.g. inositol or a derivate thereof).
The concentrated culture according to any one of the preceding claims, wherein the culture is dried (e.g. freeze-dried) and comprises from about 0.5% w/w to about 20.0% w/w sugar alcohol (e.g. inositol or a derivate thereof).
The concentrated culture according to any one of the preceding claims, wherein the culture is dried (e.g. freeze-dried) and comprises from about 0.5% w/w to about 30.0% w/w inositol, e.g. from about 1.0% w/w to about 15.0% w/w inositol, or e.g. from about 2.0% w/w to about 12.0% w/w inositol.
The concentrated culture according to any one of the preceding claims, wherein the culture comprises lactic acid bacteria and/or Micrococcaceae bacteria in a quantity of from 1.0×108 to 1.0×1012 CFU/kg, such as from 1.0×109 to 1.0×1011 CFU/kg lactic acid bacteria and/or Micrococcaceae bacteria.
The concentrated culture according to any one of the preceding claims, wherein the culture comprises lactic acid bacteria and/or Micrococcaceae bacteria in a quantity of from 2.0×109 to 5.0×1010 CFU/kg of lactic acid bacteria and/or Micrococcaceae bacteria.
The concentrated culture according to any one of the preceding claims, wherein the culture comprises a Staphylococcus specie in a quantity of from 1.0×108 to 1.0×1012 CFU/kg, such as from 1.0×109 to 1.0×1011 CFU/kg Staphylococcus specie.
The concentrated culture according to any one of the preceding claims, wherein the culture comprises a Staphylococcus specie in a quantity of from 2.0×109 to 5.0×1010 CFU/kg of Staphylococcus specie.
Use of citric acid or a salt thereof, for preserving and/or increasing the nitrate reductase activity of a bacteria having nitric acid reductase activity selected from the group consisting of lactic acid bacteria and Micrococcaceae bacteria (such as a Staphylococcus specie).
The use according to any one of claims 58 to 61, wherein the bacteria (such as a Staphylococcus species) is comprised in a concentrated culture produced by the method as defined by any one of claims 63 to 89.
A method for preserving and/or increasing the nitrate reductase activity of a bacteria having nitrate reductase activity selected from the group consisting of lactic acid bacteria and Micrococcaceae bacteria (such as e.g. a Staphylococcus species) comprising the steps of:
- a) mixing a formulation comprising citric acid or a salt thereof and a bacterium having nitrate reductase activity selected from the group consisting of lactic acid bacteria and Micrococcaceae bacteria (such as e.g. a Staphylococcus species) to obtain a mixture comprising citric acid or a salt thereof and bacteria;
- b) optionally freezing the resulting mixture of a) to obtain a frozen material;
- c) optionally drying the mixture of a), or the frozen material of step b), to obtain a dried material;
- and
- d) optionally packing the frozen material obtained from step b), or the dried material of step c) to obtain a packaged product.
The method according to claim 72, further defined by any one of the claims 63 to 71 or claims 76 to 89.
A method for making a dried concentrated culture comprising:
- a) mixing a formulation comprising a citric acid or a salt thereof and bacteria having nitrate reductase activity selected from the group consisting of lactic acid bacteria and Micrococcaceae bacteria (e.g. one or more species of Staphylococcus) having nitrate reductase activity;
- b) drying the resulting mixture of a) to obtain a dried material;
- c) optionally grinding the obtained dried material of b);
- and
- d) packing the dried material.
A method for making a frozen concentrated culture comprising:
- a) mixing a formulation comprising a citric acid or a salt thereof and bacteria having nitrate reductase activity selected from the group consisting of lactic acid bacteria and Micrococcaceae bacteria (e.g. one or more species of Staphylococcus) having nitrate reductase activity;
- b) freezing the resulting mixture of a) to obtain a frozen material;
- and
- c) packing the frozen material.
A method for making a freeze-dried concentrated culture comprising:
- a) mixing formulation comprising a citric acid or a salt thereof and bacteria having nitrate reductase activity selected from the group consisting of lactic acid bacteria and Micrococcaceae bacteria (e.g. one or more species of Staphylococcus) having nitrate reductase activity;
- b) freezing the resulting mixture of a) to obtain a frozen material;
- c) subliming water from the frozen material to freeze-dry the material of b);
- e) optionally grinding the obtained freeze-dried material of c);
- and
- f) packing the freeze-dried material.
A method for making a spray-dried concentrated culture comprising:
- a) mixing a formulation comprising a citric acid or a salt thereof and bacteria having nitrate reductase activity selected from the group consisting of lactic acid bacteria and/or Micrococcaceae bacteria (e.g. one or more species of Staphylococcus) having nitrate reductase activity;
- b) spray-drying the resulting mixture of a) to obtain a spray dried material;
- and
- c) packing the spray-dried material.
A method for making a dried concentrated culture comprising:
- a) mixing a Staphylococcus specie having nitrate reductase activity with a formulation comprising citric acid or a salt thereof;
- b) drying the resulting mixture of a) to obtain a dried material;
- c) optionally grinding the obtained dried material of b);
- and
- d) packing the dried material.
A method for making a frozen concentrated culture comprising:
- a) mixing a formulation comprising citric acid or a salt thereof and a Staphylococcus specie having nitrate reductase activity;
- b) freezing the resulting mixture of a) to obtain a frozen material;
- c) packing the frozen material.
A method for making a freeze-dried concentrated culture comprising:
- a) mixing a formulation comprising citric acid or a salt thereof and a Staphylococcus specie having nitrate reductase activity;
- b) freezing the resulting mixture of a) to obtain a frozen material;
- c) subliming water from the frozen material to freeze-dry the material of b);
- e) optionally grinding the obtained freeze-dried material of c);
- and
- f) packing the freeze-dried material.
A method for making a spray dried concentrated culture comprising:
- a) mixing a formulation comprising citric acid or salt thereof and a Staphylococcus specie having nitrate reductase activity;
- b) spray drying the resulting mixture of a) to obtain a spray dried material;
- and
- c) packing the spray dried material.
The method according to any one of the preceding claims, wherein the concentrated culture is pelletized.
The method according to any one of the preceding claims, wherein the concentrated culture is frozen in a tray.
The method according to any one of the preceding claims, wherein the concentrated culture is frozen in a tray, freeze-dried and ground.
Dependencies are inferred from the claim text.