Enzyme Variants With Improved Ester Synthase Properties
AssigneeGenomatica, Inc.
InventorsAndrew M. SHUMAKER, Bernardo M. DA COSTA, Kevin HOLDEN, Louis G. HOM, Tarah S. BARON, Noah HELMAN
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Abstract
The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
Claims16
-142. (canceled)
A variant thioesterase polypeptide comprising an amino acid sequence having at least about 90% sequence identity to SEQ ID NO:
- 51, wherein said variant Thioesterase polypeptide is genetically engineered to have at least one mutation at an amino acid position selected from the group consisting of amino acids 32, 33, 34, 38, 45, 46, 48, 49, 51, 52, 76, 81, 82, 84, 88, 112, 115, 116, 118, 119, 148, 149, 156, 159 and 164, wherein said variant thioesterase polypeptide has greater ester synthase activity compared to said corresponding wild type polypeptide.
The variant polypeptide of according to claim 1, wherein said increased ester synthase activity results in properties selected from the group consisting of increased chain lengths of fatty acid esters, decreased chain lengths of fatty acid esters, and a change in fatty ester percentage of fatty acid species.
Dependencies are inferred from the claim text.