Inducible, Tunable, and Multiplex Human Gene Regulation Using CRISPR-Cpf1

A fusion protein comprising a catalytically inactive Lachnospiraceae bacterium ND2006 Cpf1 (dLbCpf1) fused to a conditional dimerization domain, with an optional intervening linker.

A method of increasing expression of a target gene in a cell, the method comprising contacting the cell with, or expressing in the cell, one or more of:

• (i) a fusion protein comprising a catalytically inactive Lachnospiraceae bacterium ND2006 protein Cpf1 (dLbCpf1) fused to at least one activation domain, and at least one crRNA that directs the fusion protein to a regulatory region of the target gene;
• or
• (ii) a first fusion protein comprising a catalytically inactive Lachnospiraceae bacterium ND2006 Cpf1 (dLbCpf1) fused to a conditional dimerization domain, with an optional intervening linker, and a second fusion protein comprising an activation domain fused to a second conditional dimerization domain that dimerizes with the conditional dimerization domain in the first fusion protein in the presence of a dimerizing agent, with an optional intervening linker between the activation domain and the second dimerizing domain, and at least one crRNA that directs the fusion protein to a regulatory region of the target gene.