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US 2021/0309691 A1Published application
Methods and compositions for nucleoside triphosphate and ribonucleic acid production
AssigneeGreenLight Biosciences, Inc.
InventorsDrew S. Cunningham, Daniel MacEachran, James Robbins Abshire, Himanshu Dhamankar, Ifeyinwa Iwuchukwu, Mehak Gupta, Matthew Eduardo Moura, Naveen Sudharsan, Nicholas Skizim, Rachit Jain, Karthikeyan Ramachandriya
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Abstract
Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.
Claims29
A method for producing one or more nucleoside triphosphates (NTPs), comprising:
- incubating in a reaction mixture one or more nucleoside monophosphates (NMPs), at least one polyphosphate kinase (PPK), and polyphosphate.
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A method for producing one or more nucleoside triphosphates (NTPs), comprising:
- incubating in a reaction mixture one or more nucleoside diphosphates (NDPs), at least one polyphosphate kinase, and polyphosphate.
A method for producing nucleoside triphosphates (NTPs) comprising:
- (a) incubating (i) cellular RNA and (ii) a ribonuclease to produce 5′ nucleoside monophosphates (NMPs);
- (b) eliminating the ribonuclease;
- and
- (c) incubating the 5′ NMPs with at least one polyphosphate kinase (PPK), and polyphosphate to produce NTPs.
1 more element
Dependencies are inferred from the claim text.