Compositions and methods for liquid-mediated delivery of pollen
AssigneeMonsanto Technology LLC
InventorsZachary Boyer, David A. Morgenstern, Michelle R. Naert
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Abstract
The invention provides novel compositions and methods for liquid-mediated delivery of pollen to a female reproductive part of a recipient plant. The pollen suspension solutions provided include a surfactant, an oil, a solute, or an aqueous solution, and about 2% to about 20% pollen by weight. The methods provided include producing a liquid pollen suspension solution comprising pollen from a donor plant and spraying the solution onto at least a first female reproductive part of a recipient plant, thereby pollinating the female reproductive part with the pollen from the donor plant.
Claims54
A pollen suspension solution comprising:
- a) a surfactant;
- b) an oil or an aqueous solution;
- and
- c) about 2% to about 20% pollen by weight.
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A method for liquid-mediated delivery of pollen to a female reproductive part of a recipient monocot plant comprising the steps of:
- a) obtaining a liquid pollen suspension solution comprising pollen from a donor monocot plant;
- and
- b) spraying said pollen suspension solution onto at least a first female reproductive part of the recipient monocot plant, thereby pollinating the female reproductive part with the pollen from the donor monocot plant.
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A pollen suspension solution comprising:
- a) at least one solute at a concentration of about 0.5M to about 3.0M to increase the tonicity of the solution;
- and
- b) about 2% to about 20% pollen by weight;
- wherein said solute is impermeable to said pollen in said solution.
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Dependencies are inferred from the claim text.