Method for screening adulteration of fibrate anti-hyperlipidemia chemicals in tea by combined method of high performance thin layer chromatography and bioluminescence

Abstract

A method for screening adulteration of fibrate anti-hyperlipidemia chemicals in tea by combined method of high performance thin layer chromatography (HPTLC) and bioluminescence, which belongs to the field of food inspection. The method includes: firstly, formulating standard solutions of bezafibrate and ciprofibrate, and preparing a tea sample; pre-washing a thin-layer plate and then performing HPTLC spotting; performing HPTLC separation to move original mixed target objects mixed originally onto different positions of the thin-layer plate according to different molecular structures, so as to form a physical isolation; and subsequently, simultaneous detection of multiple targets in the tea sample could be realized conveniently through luminous bacteria coupled with the thin-layer plate by an immersed manner. The present disclosure establishes a method capable of detecting anti-hyperlipidemia chemicals in tea rapidly and quantitatively by the combined detection method of HPTLC and bioluminescence, which has the advantages of being economic, rapid, simple and convenient.

Dependencies are inferred from the claim text.