Biotechnological production of cannabinoids
AssigneeTechnische Universitaet Dortmund
InventorsOliver KAYSER, Felix-Oliver STEHLE
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Abstract
A method for the recombinant production of cannabigerolic acid in a host organism may use a modified prenyltransferase. A modified prenyltransferase, a nucleic acid molecule that codes for the modified prenyltransferase, and a recombinant organism that includes the modified prenyltransferase and/or the nucleic acid are also disclosed here.
Claims18
A method for recombinant production of cannabigerolic acid in a host organism, the method comprising:
- introducing into the host organism a nucleic acid molecule which comprises a first heterologous nucleotide sequence which encodes a modified prenyltransferase, wherein the prenyltransferase being modified comprises a substrate specificity extended to olivetolic acid, and wherein the product ratio of cannabigerolic acid:2-O-geranyl olivetolic acid is at least 1:1 upon reaction of olivetolic acid with geranyl diphosphate;
- culturing the host organism under conditions to express the nucleotide sequence for encoding the modified prenyltransferase;
- culturing the host organism under conditions to produce cannabigerolic acid.
A prenyltransferase comprising an amino acid sequence having at least one amino acid substitution with respect to the amino acid sequence specified in SEQ ID NO:
- 2 and which has at least 80% sequence identity with the amino acid sequence specified in SEQ ID NO:
- 2 over the entire length thereof.
Dependencies are inferred from the claim text.