Cho cell line, construction thereof and recombinant protein expression system using cho cell line

Abstract

The disclosure relates to genetic engineering, and more particularly to a CHO cell line, its construction and a recombinant protein expression system using the CHO cell line. Regardless of the presence screening pressure, the recombinant protein expression system constructed by the 60th passage of the recombinant APRT gene-deficient CHO cell line is much higher than the normal recombinant CHO cell in the expression retention of the target protein EGFP. In the absence of G418 screening pressure, the expression level of the recombinant vitronectin in the recombinant protein expression system constructed by the 30th passage of the recombinant APRT gene-deficient CHO cell is significantly higher than the expression level of the corresponding target protein in the normal recombinant CHO cells.

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