Generation of acyl amino acids
AssigneeModular Genetics, Inc.
InventorsKevin A. Jarrell, Michelle Pynn
Life of this patent
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Abstract
Engineered polypeptides or engineered microbial cells useful in synthesizing acyl amino acids are provided. In some embodiments, engineered polypeptides or engineered microbial cells are useful in synthesizing acyl amino acids with one or more hydroxyl and/or methyl groups at one or more positions of the fatty acid portion of the acyl amino acid (e.g., at ω-1, ω-2, and/or ω-3 positions of the fatty acid portion of the acyl amino acid). Also provided are methods of making acyl amino acids using engineered polypeptides and/or engineered microbial cells.
Claims70
An engineered microbial cell:
- (i) expresses at least one peptide synthetase, which peptide synthetase synthesizes an acyl amino acid, and
- (ii) comprises a modification that modulates hydroxylation and/or methylation of a fatty acid portion of the acyl amino acid.
A method comprising a step of:
- culturing a microbial cell in the presence of a medium comprising a carbon source, a fatty acid, and amino acid under conditions and for a time sufficient for an acyl amino acid composition to be made, wherein the microbial cell comprises a modification that modulates hydroxylation and/or methylation of a fatty acid portion of the acyl amino acid.
A composition comprising
- (a) a microbial cell;
- and
- (b) an acyl glycinate comprising a fatty acid covalently linked to amino acid,
- wherein the fatty acid (i) is a straight chain fatty acid and/or (ii) consists of an even number of carbons.
A method comprising a step of:
- culturing a microbial cell in the presence of a medium comprising a carbon source, a fatty acid, and amino acid and in the presence of an enzyme that covalently attaches a fatty acid to an amino acid under conditions and for a time sufficient for an acyl amino acid composition to be made, wherein the microbial cell comprises a modification that modulates hydroxylation and/or methylation of a fatty acid portion of the acyl amino acid.
Dependencies are inferred from the claim text.