Back to results
US 2021/0285016 A1Published application
Methods of making and using e,z,e-geranylgeranyl diphosphate
AssigneeWiSys Technology Foundation, Inc.
InventorsFrancis Michelle Mann, Leah C. Poulos
Life of this patent
Estimating the term…
Abstract
Methods of making and using E,Z,E-GGPP are provided. Also provided are expression cassettes encoding isoprenyl diphosphate synthases, e.g., cis- or trans-isoprenyl diphosphate synthases.
Claims20
A compound having the structure (11):
- wherein
- n is 1 or an integer from 2 to 20 so as to represent a number of repeating groups each instance of which independently has a stereochemistry of E or Z;
- Q is a phosphate, a phosphonate, X, OH, —OX, halide, alkylsulfonyl, or arylsulfonyl;
- Y is H or a phosphonate;
- X is a phosphonate, an ester, or a biomolecule;
- and
- R is H, alkyl, or a dimer group;
- or a salt thereof.
5 more elements
A host cell expressing at least one heterologous isoprenyl diphosphate synthase having at least 80% amino acid sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3.
An in vitro method to produce E,Z,E-GGPP, comprising:
- combining at least one isolated trans-isoprenyl diphosphate synthase having at least 80% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:3, and at least two substrates therefore, or combining at least one isolated cis-isoprenyl diphosphate synthase having at least 80% amino acid sequence identity to SEQ ID NO:1, at least one isolated trans-isoprenyl diphosphate synthase having at least 80% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:3, and a substrate for the cis-isoprenyl diphosphate synthase, in an amount effective to produce E,Z,E-GGPP.
Dependencies are inferred from the claim text.