# US 2021/0280406 A1 · General-purpose nanochip for mass spectrum analysis, preparation method therefor, and application thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0280406 A1 - Document ID: US20210280406A1 - Status: Published application - Kind: A1 - Application number: 17/303,218 - Filed: 2021-05-24 - Published: 2021-09-09 - Earliest claimed: 2018-11-23 - Assignees: Hangzhou Well-Healthcare Technologies Co., LTD - Applicants: Hangzhou Well-Healthcare Technologies Co., LTD - Inventors: Jianmin WU; Xiaoming CHEN; Xisheng CHEN; Qiaoling ZHONG; Chunyan LUAN; Jiekai YU - Web page: https://patentgrep.com/patent/US20210280406A1 ## CPC - H01J 49/0418: {for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates} - H01J 49/0009: {Calibration of the apparatus} - B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) - B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES ## Abstract A universal nanochip for mass spectrometry analysis and preparing method and application of the same, relates to a technical field of mass spectrometry analysis. A main material of the nanochip is a silicon-based semiconductor material, array-type spotting wells are distributed at a surface of the main material, and an inner surface of the spotting well is of a nanostructure; the surface of the main material has a regional hydrophobic modification, and inside the array-type spotting well is a hydrophilic region and outside the spotting well is a hydrophobic region; or outside the array-type spotting well is a hydrophilic region and inside the spotting well is a hydrophobic region. The nanostructure can extract molecules on a surface of a biological tissue sample to be tested, and improves laser energy absorption and utilization, thereby improving ionization efficiency and enhancing mass spectrum signals. The universal nanochip can be widely applied to clinical inspection. ## Claims ### Claim 1 A universal nanochip for mass spectrometry analysis, wherein a main material of the nanochip is a silicon-based semiconductor material, array-type spotting wells are distributed at a surface of the main material, and an inner surface of the spotting well is of a nanostructure; the surface of the main material has a regional hydrophobic modification, and inside the array-type spotting well is a hydrophilic region and outside the spotting well is a hydrophobic region; or outside the array-type spotting well is a hydrophilic region and inside the spotting well is a hydrophobic region, and the nanochip is used for detecting a substance of molecular weight<1000 Da or 1-10 KDa or 2-20 KDa or 2-10 KDa. ### Claim 2 (depends on 1) The universal nanochip for mass spectrometry analysis according to claim 1, wherein a thickness of the nanostructure is 0.2 to 5 μm. ### Claim 3 (depends on 1) The universal nanochip for mass spectrometry analysis according to claim 1, wherein the nanostructure is selected from any one or more of nanowires, nanofibers, nanocolumns, nanopyramids, nanoparticles, and nanoporous. ### Claim 4 (depends on 1) The universal nanochip for mass spectrometry analysis according to claim 1, wherein the silicon-based semiconductor material is selected from any one or more of single-crystal silicons, polycrystalline silicons, silicon-based epitaxial metals, silicon-based epitaxial nonmetallic elemental substances, and silicon-based epitaxial oxides. ### Claim 5 (depends on 1) The universal nanochip for mass spectrometry analysis according to claim 1, wherein a surface modification of the hydrophobic region is made by chemical vapor deposition or liquid phase chemical modification, and an adopted reagent is selected from any one or more of silanes, siloxanes, thiols, and terminal olefins. ### Claim 6 (depends on 1) The universal nanochip for mass spectrometry analysis according to claim 1, wherein the array-type spotting wells are round or square in shape. ### Claim 7 (depends on 1) The universal nanochip for mass spectrometry analysis according to claim 1, wherein the nano structure has a tip. ### Claim 8 (depends on 1) A preparing method of the universal nanochip for mass spectrometry analysis according to claim 1, comprising following steps: S1: scribing a main material, wherein the main material is scribed using a laser or a grinding wheel in a clean room; S2: cleaning the main material, wherein the scribed main material is placed in a concentrated sulfuric acid/hydrogen peroxide mixed solution for an ultrasonic cleaning, washing the solution on a surface of the main material with deionized water; and placing sequentially the main material into an ethanol solution and an isopropanol solution for an ultrasonic cleaning; S3: patterning the array-type spotting well, wherein any one or more of metal stamping, lithography, a blue film method and screen printing are adopted to realize a patterning design on the surface of the main material; S4: constructing a nanostructure on an inner surface of the array-type spotting well, wherein the nanostructure is prepared at a position corresponding to the array-type spotting well by using any one or more of reactive ion etching, chemical vapor deposition, physical vapor deposition, atomic layer deposition, wet chemical etching, a template method, a hydrothermal method and drop coating according to the pattern on the surface of the main material; and S5: hydrophobically modifying the surface of the main material. ### Claim 9 (depends on 1) An application of the universal nanochip for mass spectrometry analysis according to claim 1, comprising following aspects: rapid clinical identification of microorganisms; rapid detection of small molecule metabolites in biological samples; drug sensitivity testing of antibiotics; rapid mass spectrometry imaging of tissue samples; SNP gene detection; detection of protein and peptide in serum. ### Claim 10 (depends on 1) The application of the universal nanochip for mass spectrometry analysis according to claim 1, wherein the universal nanochip is applicated in the rapid detection of small molecule metabolites in biological samples, a variation rate of peak positions between the wells of the nanochip is below 600 ppm.