# US 2021/0278422 A1 · Reagent and method for measuring hemoglobins

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- Number: US 2021/0278422 A1
- Document ID: US20210278422A1
- Status: Published application
- Kind: A1
- Application number: 17/256,919
- Filed: 2019-09-05
- Published: 2021-09-09
- Earliest claimed: 2018-09-12
- Assignees: Sekisui Medical Co., Ltd.; Tokuyama Sekisui Co., Ltd.
- Applicants: Sekisui Medical Co., Ltd.; Tokuyama Sekisui Co., Ltd.
- Inventors: Kazuyuki Oishi; Hiroaki Taira; Tomonori Inoue; Ryo Takemoto
- Web page: https://patentgrep.com/patent/US20210278422A1

## CPC

- G01N 33/721: {Haemoglobin}
- B01D 15/362: {Cation-exchange}
- G01N 30/06: Preparation
- G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N 2030/8822: {involving blood}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)

## Abstract

Provided is a reagent for measuring hemoglobins capable of satisfactorily causing hemolysis and measuring hemoglobins with high accuracy. The reagent for measuring hemoglobins according to the present invention is a reagent for measuring hemoglobins used for measuring hemoglobins by cation exchange liquid chromatography, including a specific nonionic surfactant or a specific amphoteric surfactant.

## Claims

### Claim 1

A reagent for measuring hemoglobins used for measuring hemoglobins by cation exchange liquid chromatography, comprising:
a nonionic surfactant or an amphoteric surfactant,
the nonionic surfactant being Component A1 below, Component A2 below, Component A3 below, Component A4 below, Component A5 below, Component A6 below, Component A7 below, Component A8 below, or Component A9 below, and
the amphoteric surfactant being Component B1 below, Component B2 below, or Component B3 below:
Component A1: Polyoxyethylene alkyl ether having an average number of moles of an oxyethylene group added of 8 or more and 20 or less, and a carbon number of an alkyl group of 12 or more and 17 or less
Component A2: Polyoxyethylene stearyl ether having an average number of moles of an oxyethylene group added of 40 or more and 60 or less
Component A3: Polyoxyethylene oleyl ether having an average number of moles of an oxyethylene group added of 9 or more and 15 or less
Component A4: Polyoxyethylene-sec-alkyl ether having an average number of moles of an oxyethylene group added of 6 or more and 10 or less, and a carbon number of an alkyl group of 11 or more and 15 or less
Component A5: Polyoxyethylene tridecyl ether having an average number of moles of an oxyethylene group added of 15
Component A6: Polyoxyethylene polyoxypropylene alkyl ether having an HLB value of 11 or more and 15 or less
Component A7: n-nonanoyl-N-methyl-D-glucamine
Component A8: n-octyl-β-D-glucopyranoside
Component A9: Saponin
Component B1: 3-[(3-cholamidopropyl)dimethylammonio]propane sulfonate
Component B2: 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxypropane sulfonate
Component B3: Lauryl dimethylamine oxide.

### Claim 2 (depends on 1)

The reagent for measuring hemoglobins according to claim 1, comprising the nonionic surfactant,
the nonionic surfactant including the Component A1, the Component A2, the Component A3, the Component A4, the Component A5, or the Component A6,
the Component A1 being polyoxyethylene lauryl ether having an average number of moles of an oxyethylene group added of 9, 12, or 19, or polyoxyethylene cetyl ether having an average number of moles of an oxyethylene group added of 13 or 20,
the Component A2 being polyoxyethylene stearyl ether having an average number of moles of an oxyethylene group added of 50,
the Component A3 being polyoxyethylene oleyl ether having an average number of moles of an oxyethylene group added of 9 or 13,
the Component A4 being polyoxyethylene-sec-alkyl ether having an average number of moles of an oxyethylene group added of 7 or 9 and a carbon number of an alkyl group of 11 or more and 15 or less, and
the Component A6 being polyoxyethylene polyoxypropylene alkyl ether having an HLB value of 12.5, 12.7, or 14.0.

### Claim 3 (depends on 1)

The reagent for measuring hemoglobins according to claim 1, comprising the nonionic surfactant,
the nonionic surfactant including the Component A1 or the Component A3,
the Component A1 being polyoxyethylene alkyl ether having an average number of moles of an oxyethylene group added of 10 or more and 15 or less, and a carbon number of an alkyl group of 12 or more and 17 or less, and
the Component A3 being polyoxyethylene oleyl ether having an average number of moles of an oxyethylene group added of 10 or more and 15 or less.

### Claim 4 (depends on 1)

The reagent for measuring hemoglobins according to claim 1,
the Component A1 being polyoxyethylene lauryl ether having an average number of moles of an oxyethylene group added of 12, or polyoxyethylene cetyl ether having an average number of moles of an oxyethylene group added of 13, and
the Component A3 being polyoxyethylene oleyl ether having an average number of moles of an oxyethylene group added of 13.

### Claim 5 (depends on 1)

The reagent for measuring hemoglobins according to claim 1, comprising the nonionic surfactant,
the reagent for measuring hemoglobins having a content of the nonionic surfactant of 0.010% by weight or more and 1.0% by weight or less.

### Claim 6 (depends on 1)

The reagent for measuring hemoglobins according to claim 1, comprising the amphoteric surfactant,
the reagent for measuring hemoglobins having a content of the amphoteric surfactant of 0.01% by weight or more and 1.0% by weight or less.

### Claim 7 (depends on 1)

A method for measuring hemoglobins, comprising the steps of:
mixing an erythrocyte-containing sample and the reagent for measuring hemoglobins according to claim 1 to obtain a mixed solution; and
measuring the mixed solution by cation exchange liquid chromatography.