Redox-labile fluorescent probes and their surface immobilization methods for the detection of metabolites
AssigneeThe Regents of the University of California
InventorsWei Wei, Min Xue, Zhonghan Li, Hanjun Cheng
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Abstract
In various embodiments an analytical method for profiling lactate (or other analyte) production in single cells, via the use of coupled enzyme reactions on surface-grafted resazurin/resofurin molecules is provided. Additionally surface-bound resazurin/resofurin that retains redox indicator activity as well as microfluidic devices comprising such sur-face-bound resazurin/resofurin indicators are provided.
Claims138
A detection reagent comprising a redox-active resazurin attached to a solid support.
The detection reagent of claim said 9, wherein said diarylcyclooctyne moiety comprises dibenzylcyclooctyne (DBCO).
Test agent comprises one or more anti-cancer drugs.
A modified resazurin functionalized for attachment to a substrate while preserving the oxidation-reduction indicator activity of said resazurin when attached to said substrate.
The modified resazurin of claim said 124, wherein said diarylcyclooctyne moiety comprises dibenzylcyclooctyne (DBCO).
Dependencies are inferred from the claim text.