# US 2021/0278322 A1 · Digital Dispense System

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- Number: US 2021/0278322 A1
- Document ID: US20210278322A1
- Status: Published application
- Kind: A1
- Application number: 17/328,258
- Filed: 2021-05-24
- Published: 2021-09-09
- Earliest claimed: 2019-01-04
- Assignees: Funai Electric Co., Ltd.
- Applicants: Funai Electric Co., Ltd.
- Inventors: Bruce A Deboard; Michael A. Marra, III; Pramod K. SHARMA
- Web page: https://patentgrep.com/patent/US20210278322A1

## CPC

- G01N 1/312: {for samples mounted on planar substrates}
- B01L 3/0203: {Burettes, i.e. for withdrawing and redistributing liquids through different conduits}
- B01L 3/0265: {using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves}
- B01L 3/0268: {using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries}
- G01F 11/006: {Details or accessories (general details G01F15/00)}
- G01N 1/2813: {Producing thin layers of samples on a substrate, e.g. smearing, spinning-on (G01N1/30 takes precedence)}
- G01N 35/00029: {provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence)}
- G01N 35/00722: {Communications; Identification}
- G01N 35/1016: {Control of the volume dispensed or introduced}
- G01N 35/109: {with two horizontal degrees of freedom}
- G01N 2001/2826: {Collecting by adsorption or absorption}
- G01N 2001/317: {spraying liquids onto surfaces}
- G01N 2035/00039: {Transport arrangements specific to flat sample substrates, e.g. pusher blade}
- G01N 2035/00138: {Slides}
- G01N 2035/00891: {Displaying information to the operator}
- G01N 2035/1041: {Ink-jet like dispensers}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01F: MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME

## Abstract

A digital dispense system and method for analyzing samples. The system includes a fluid droplet ejection system housed in a compact housing unit. The fluid droplet ejection system contains a fluid droplet ejection head and fluid cartridge containing one or more fluids to be dispensed, a cartridge translation mechanism for moving the fluid droplet ejection head and fluid cartridge back and forth over a sample holder in an x direction; and a sample holder translation mechanism for moving a sample back and forth beneath the fluid droplet ejection head and fluid cartridge in a y direction orthogonal to the x direction. A digital display device is attached to the fluid droplet ejection system for displaying fluid volume information to a user. The fluid volume information is selected from relative fluid volume, absolute fluid volume, and a combination of relative and absolute fluid volumes.

## Claims

### Claim at position 1

1-6. (canceled)

### Claim 7

A method for staining slides without dipping or immersing slides in a dye, comprising:
providing a digital fluid droplet ejection system housed in a compact housing unit, the fluid droplet ejection system including:
a fluid droplet ejection head and fluid cartridge containing one or more fluids to be dispensed,
a cartridge translation device for moving the fluid droplet ejection head and fluid cartridge back and forth over a slide holder in an x direction, and
a slide holder translation device for moving one or more slides back and forth beneath the fluid droplet ejection head and fluid cartridge in a y direction orthogonal to the x direction;
attaching a digital display device to the digital fluid droplet ejection system;
ejecting fluid from the fluid droplet ejection head and fluid cartridge in one or more locations on the slide while moving the fluid cartridge in the x direction and moving the one or more slides in the y direction; and
displaying fluid volume information to a user on the digital display device, wherein the fluid volume information is selected from the group consisting of relative fluid volume, absolute fluid volume, and a combination of relative and absolute fluid volumes.

### Claim 8 (depends on 7)

The method of claim 7, further comprising ejecting two or more fluids on the slides simultaneously.

### Claim 9 (depends on 7)

The method of claim 7, further comprising ejecting two or more fluids on the slides sequentially.

### Claim 10 (depends on 7)

The method of claim 7, wherein the fluid volume information is displayed by a bar graph representation of fluid in a particular location on a slide.

### Claim 11 (depends on 7)

The method of claim 7, wherein the digital display device comprises a relative volume graphic for each fluid dispensed to a slide location on a slide.

### Claim 12 (depends on 7)

The method of claim 7, wherein the digital display devices comprises an absolute volume graphic of fluid dispensed and a relative volume graphic for each fluid dispensed to a slide location on a slide.

### Claim 13 (depends on 7)

The method of claim 7, wherein the fluid droplet ejection system comprises a processor and a memory, further comprising storing fluid droplet information in the memory and transferring the fluid droplet information to the digital display device via the processor.

### Claim 14 (depends on 7)

The method of claim 7, wherein the digital display device comprises a portable or laptop computer.

### Claim 15

A method for dispensing fluid into wells of a micro-well plate, the method comprising:
providing a digital fluid droplet ejection system housed in a compact housing unit, the fluid droplet ejection system including:
a fluid droplet ejection head and fluid cartridge containing one or more fluids to be dispensed,
a cartridge translation device for moving the fluid droplet ejection head and fluid cartridge back and forth over a micro-well plate holder in an x direction, and
a micro-well plate holder translation device for moving a micro-well plate back and forth beneath the fluid droplet ejection head and fluid cartridge in a y direction orthogonal to the x direction;
attaching a digital display device to the digital fluid droplet ejection system;
ejecting fluid from the fluid droplet ejection head and fluid cartridge into one or more wells of the micro-well plate while moving the fluid cartridge in the x direction and moving the micro-well plate in the y direction; and
displaying fluid volume information to a user on the digital display device, wherein the fluid volume information is selected from the group consisting of relative fluid volume, absolute fluid volume, and a combination of relative and absolute fluid volumes.

### Claim 16 (depends on 15)

The method of claim 15, wherein the fluid volume information is displayed by a bar graph representation of fluid in the one or more wells of the micro-well plate.

### Claim 17 (depends on 15)

The method of claim 15, wherein the fluid volume information comprises a graphic for each fluid dispensed into the one or more wells in the micro-well plate.

### Claim 18 (depends on 15)

The method of claim 15, wherein the fluid volume information comprises a graphic of fluid dispensed and a relative volume graphic for each fluid dispensed into the one or more wells of the micro-well plate.

### Claim 19 (depends on 15)

The method of claim 15, wherein the fluid droplet ejection system comprises a processor and a memory for storing fluid droplet information and for transferring the fluid droplet information to the digital display device.

### Claim 20 (depends on 15)

The method of claim 15, wherein the digital display device comprises a portable or laptop computer.
