# US 2021/0278319 A1 · Sample extraction support tool and sample extraction method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0278319 A1 - Document ID: US20210278319A1 - Status: Published application - Kind: A1 - Application number: 17/255,489 - Filed: 2019-04-25 - Published: 2021-09-09 - Earliest claimed: 2018-07-03 - Assignees: Shimadzu Corporation - Applicants: Shimadzu Corporation - Inventors: Ippei TAKEUCHI - Web page: https://patentgrep.com/patent/US20210278319A1 ## CPC - B01L 3/5021: {Test tubes specially adapted for centrifugation purposes} - G01N 1/02: Devices for withdrawing samples {(sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08)} - B01L 2300/0609: Holders integrated in container to position an object - B01L 2300/0829: Multi-well plates; Microtitration plates - B01L 2300/0861: Configuration of multiple channels and/or chambers in a single devices - B01L 2400/0409: centrifugal forces - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The sample extraction support tool is placed on an extraction container having at least one well for accommodating a sample extracted from a test piece holding the sample in a microchannel provided therein with both ends open. The sample extraction support tool includes at least one test piece holding portion having an opening for guiding a sample extracted from the test piece to a bottom of the well and configured to hold the test piece at a position where the test piece is not in contact with the sample which is extracted from the test piece and is stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom of the well. ## Claims ### Claim 1 A sample extraction support tool which is used while being placed on an extraction container having at least one well for accommodating a sample extracted from a test piece holding the sample in a microchannel provided therein with both ends open, wherein the sample extraction support tool comprises at least one test piece holding portion, the test piece holding portion has an opening for guiding the sample extracted from the test piece to a bottom of the well, and is configured to hold the test piece at a position where the test piece is not in contact with the sample which is extracted from the test piece and is stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom surface of the well. ### Claim 2 (depends on 1) The sample extraction support tool according to claim 1, wherein the opening is provided at a lower end of the test piece holding portion and has a size not allow the test piece to pass through, and at least part of an inner surface of the test piece holding portion is inclined so as to guide the sample extracted from the test piece to the opening. ### Claim 3 (depends on 1) The sample extraction support tool according to claim 1, wherein the extraction container is a well-plate in which a plurality of the wells are provided on a same plane, and the sample extraction support tool is a plate-shaped member having the test piece holding portions corresponding to each of the wells of the extraction container. ### Claim 4 (depends on 2) The sample extraction support tool according to claim 2, wherein the extraction container is a well-plate in which a plurality of the wells are provided on a same plane, and the sample extraction support tool is a plate-shaped member having the test piece holding portions corresponding to each of the wells of the extraction container. ### Claim 5 A sample extraction method comprising: a support tool placing step of placing, on an extraction container having at least one well for accommodating a sample extracted from a test piece holding the sample in a microchannel provided therein with both ends open, a sample extraction support tool including at least one test piece holding portion, the test piece holding portion having an opening for guiding a sample extracted from the test piece to a bottom of the well and configured to hold the test piece at a position where the test piece is not in contact with a sample which is extracted from the test piece and stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom of the well; a piece setting step of causing the test piece holding portion of the sample extraction support tool to hold the test piece; and a sample extracting step of extracting a sample from the test piece to the bottom of the well by swiveling the extraction container so that a centrifugal force acts in a direction from a top side of the well to a bottom side of the well of the extraction container, in this order. ### Claim 6 (depends on 5) The sample extraction method according to claim 5, further comprising: a test piece removing step of removing the test piece from the well of the extraction container by moving the sample extraction support tool from above the extraction container after the sample extracting step. ### Claim 7 (depends on 5) The sample extraction method according to claim 5, wherein the opening of the sample extraction support tool is provided at a lower end of the test piece holding portion and has a size not allow the test piece to pass through, and at least part of an inner surface of the test piece holding portion is inclined so as to guide the sample extracted from the test piece to the opening. ### Claim 8 (depends on 5) The sample extraction method according to claim 5, wherein the extraction container is a well-plate in which a plurality of the wells are provided on a same plane, and the sample extraction support tool is a plate-shaped member having the test piece holding portions corresponding to each of the wells of the extraction container.