Enzymetic preparation of glucosamine
AssigneeTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
InventorsChun YOU, Dongdong MENG
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Abstract
A method for preparing glucosamine includes the steps of converting fructose-6-phosphate (F6P) and an ammonium salt to glucosamine-6-phosphate (GlcN6P) under the catalysis of glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and producing glucosamine (GlcN) by the dephosphorylation of GlcN6P under the catalysis of an enzyme capable of catalyzing the dephosphorylation. Such a method can be used to prepare glucosamine by in vitro enzymatic biosystem.
Claims10
A method for preparing glucosamine by using an in vitro enzymatic reaction comprising:
- converting fructose-6-phosphate (F6P) and an ammonium salt to glucosamine-6-phosphate (GlcN6P) under the catalysis of a glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD);
- and producing glucosamine (GlcN) by the dephosphorylation of GlcN6P under the catalysis of an enzyme capable of catalyzing the dephosphorylation;
- preferably, the ammonium salt is one selected from the group consisting of ammonium sulfate, ammonium chloride, ammonium bisulfate, ammonium nitrate, ammonium carbonate, and ammonium bicarbonate, or any mixture of two or more selected from the group.
Use of a glucosamine-6-phosphate deaminase and an enzyme capable of catalyzing the dephosphorylation in the preparation of glucosamine, preferably the use in catalyzing F6P to produce glucosamine;
- preferably, the enzyme capable of catalyzing the dephosphorylation is a glucosamine-6-phosphate phosphatase.
Use of an enzyme in the preparation of glucosamine, preferably the use in catalyzing glucosamine-6-phosphate (GlcN6P) to produce glucosamine (GlcN), wherein the enzyme is encoded by a nucleotide comprising a nucleotide sequence having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 99% or 100% sequence identity with SEQ ID NO:
- 1;
- preferably, the nucleotide is a nucleotide sequence having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 99% or 100% sequence identity with SEQ ID NO:
- 1;
- preferably, the enzyme comprises an amino acid sequence having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 99% or 100% sequence identity with SEQ ID NO:
- 2;
- preferably, the enzyme has an amino acid sequence having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 99% or 100% sequence identity with SEQ ID NO:
- 2;
- preferably, the enzyme is a sugar phosphatase derived from Thermococcus kodakarensis (UniProt No. Q51145).
Dependencies are inferred from the claim text.