US 2021/0277427 A1 · METHOD FOR ACETATE CONSUMPTION DURING ETHANOLIC FERMENTATION OF CELLULOSIC FEEDSTOCKS

Abstract

The present invention provides for novel metabolic pathways to detoxify biomass-derived acetate via metabolic conversion to ethanol, acetone, or isopropanol. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more first engineered metabolic pathways to achieve: (1) conversion of acetate to ethanol; (2) conversion of acetate to acetone; or (3) conversion of acetate to isopropanol; and one or more native and/or heterologous enzymes that function in one or more second engineered metabolic pathways to produce an electron donor used in the conversion of acetate to less inhibitory compounds; wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated.

Claims16

Dependent claims

A recombinant yeast microorganism comprising an engineered metabolic pathway to convert acetate to ethanol comprising

(i) the conversion of acetate to acetyl-CoA and
(ii) the conversion of acetyl-CoA to ethanol,
wherein said microorganism expresses a heterologous enzyme that is an NADPH-specific alcohol dehydrogenase and wherein said microorganism expresses a native and/or heterologous enzyme that is encoded by a zwf1 polynucleotide recombinantly introduced into said microorganism.

Dependencies are inferred from the claim text.

Method for acetate consumption during ethanolic fermentation of cellulosic feedstocks

Life of this patent

Method for acetate consumption during ethanolic fermentation of cellulosic feedstocks

Life of this patent

Abstract

Claims16