# US 2021/0277413 A1 · Nucleic acid molecules that confer resistance to coleopteran pests

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- Number: US 2021/0277413 A1
- Document ID: US20210277413A1
- Status: Published application
- Kind: A1
- Application number: 15/770,379
- Filed: 2016-10-20
- Published: 2021-09-09
- Earliest claimed: 2015-10-28
- Assignees: Dow AgroSciences LLC
- Applicants: Dow AgroSciences LLC
- Inventors: Kenneth E. Narva; Huarong Li; Chaoxian Geng; Ignacio Mario Larrinua; Navin Elango; Aaron T. Woosley; Monica B. Olson; Matthew J. Henry; Murugesan Rangasamy; Kanika Arora; Premchand Gandra; Sarah E. Worden
- Web page: https://patentgrep.com/patent/US20210277413A1

## CPC

- C12N 15/8286: {for insect resistance}
- C07K 14/43563: {from insects}
- C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

## Claims

### Claim 1

An isolated nucleic acid comprising at least one polynucleotide operably linked to a heterologous promoter, wherein the polynucleotide is selected from the group consisting of:
SEQ ID NO:1; the complement of SEQ ID NO:1; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1; a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1; the complement of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:3-7; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:3-7; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:3-7; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:3-7;
SEQ ID NO:8; the complement of SEQ ID NO:8; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:8; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:8; a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8; the complement of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:10-12; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:10-12; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:10-12; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:10-12;
SEQ ID NO:13; the complement of SEQ ID NO:13; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:13; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:13; a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13; the complement of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:15-16; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:15-16; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:15-16; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:15-16;
SEQ ID NO:17; the complement of SEQ ID NO:17; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:17; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:17; a native coding sequence of a coleopteran organism comprising SEQ ID NO:17; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:17; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:17; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:17; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:19-20; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:19-20; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:19-20; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:19-20;
SEQ ID NO:21; the complement of SEQ ID NO:21; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:21; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:21; a native coding sequence of a coleopteran organism comprising SEQ ID NO:21; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:21; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:21; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:21; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:23-25; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:23-25; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:23-25; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:23-25;
SEQ ID NO:26; the complement of SEQ ID NO:26; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:26; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:26; a native coding sequence of a coleopteran organism comprising SEQ ID NO:26; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:26; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:26; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:26; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:28-29; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:28-29; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:28-29; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:28-29;
SEQ ID NO:30; the complement of SEQ ID NO:30; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:30; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:30; a native coding sequence of a coleopteran organism comprising SEQ ID NO:30; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:30; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:30; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:30; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:32-34; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:32-34; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:32-34; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:32-34;
SEQ ID NO:35; the complement of SEQ ID NO:35; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:35; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:35; a native coding sequence of a coleopteran organism comprising SEQ ID NO:35; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:35; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:35; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:35; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:37-39; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:37-39; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:37-39; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:37-39;
SEQ ID NO:40; the complement of SEQ ID NO:40; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:40; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:40; a native coding sequence of a coleopteran organism comprising SEQ ID NO:40; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:40; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:40; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:40; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:42-44; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:42-44; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:42-44; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:42-44;
SEQ ID NO:45; the complement of SEQ ID NO:45; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:45; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1; a native coding sequence of a coleopteran organism comprising SEQ ID NO:45; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:45; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:45; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:45; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:47-49; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:47-49; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:47-49; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:47-49;
SEQ ID NO:50; the complement of SEQ ID NO:50; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:50; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:50; a native coding sequence of a coleopteran organism comprising SEQ ID NO:50; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:50; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:50; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:50; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:52-53; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:52-53; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:52-53; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:52-53;
SEQ ID NO:54; the complement of SEQ ID NO:54; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:54; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:54; a native coding sequence of a coleopteran organism comprising SEQ ID NO:54; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:54; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:54; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:54; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:56-58; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:56-58; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:56-58; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:56-85;
SEQ ID NO:59; the complement of SEQ ID NO:59; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:59; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:59; a native coding sequence of a coleopteran organism comprising SEQ ID NO:59; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:59; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:59; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:59; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:61-63; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:61-63; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:61-63; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:61-63;
SEQ ID NO:64; the complement of SEQ ID NO:64; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:64; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:64; a native coding sequence of a coleopteran organism comprising SEQ ID NO:64; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:64; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:64; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:64; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:66-67; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:66-67; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:66-67; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:66-67;
SEQ ID NO:68; the complement of SEQ ID NO:68; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:68; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:68; a native coding sequence of a coleopteran organism comprising SEQ ID NO:68; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:68; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:68; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:68; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:70-71; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:70-71; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:70-71; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:70-71;
SEQ ID NO:72; the complement of SEQ ID NO:72; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:72; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:72; a native coding sequence of a coleopteran organism comprising SEQ ID NO:72; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:72; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:72; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:72; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:74-75; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:74-75; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:74-75; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:74-75;
SEQ ID NO:76; the complement of SEQ ID NO:76; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:76; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:76; a native coding sequence of a coleopteran organism comprising SEQ ID NO:76; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:76; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:76; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:76; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:78-79; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:78-79; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:78-79; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:78-79;
SEQ ID NO:80; the complement of SEQ ID NO:80; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:80; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:80; a native coding sequence of a coleopteran organism comprising SEQ ID NO:80; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:80; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:80; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:80; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:82-85; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:82-85; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:82-85; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:82-85;
SEQ ID NO:86; the complement of SEQ ID NO:86; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:86; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:86; a native coding sequence of a coleopteran organism comprising SEQ ID NO:86; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:86; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:86; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:86; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:88-89; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:88-89; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:88-89; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:88-89;
SEQ ID NO:90; the complement of SEQ ID NO:90; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:90; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:90; a native coding sequence of a coleopteran organism comprising SEQ ID NO:90; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:90; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:90; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:90; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:92-93; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:92-93; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:92-93; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:92-93;
SEQ ID NO:94; the complement of SEQ ID NO:94; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:94; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:94; a native coding sequence of a coleopteran organism comprising SEQ ID NO:94; the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:94; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:94; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:94; native coding sequence of a Coleopteran organism comprising SEQ ID NOs:96-97; the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:96-97; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:96-97; and the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:96-97.

### Claim 2 (depends on 1)

The polynucleotide of claim 1, wherein the polynucleotide is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:78, SEQ ID NO:79, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:83, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:90, SEQ ID NO:92, SEQ ID NO:93, SEQ ID NO:94, SEQ ID NO:96, SEQ ID NO:97, and the complements of any of the foregoing.

### Claim 3 (depends on 1)

A plant transformation vector comprising the polynucleotide of claim 1.

### Claim 4 (depends on 1)

The polynucleotide of claim 1, wherein the organism is selected from the group consisting of D. v. virgifera LeConte (western corn rootworm, “WCR”); D. barberi Smith and Lawrence (northern corn rootworm, “NCR”); D. u. howardi Barber (southern corn rootworm, “SCR”); D. v. zeae Krysan and Smith (Mexican corn rootworm, “MCR”); D. balteata LeConte; D. u. tenella; D. speciosa Germar; and D. u. undecimpunctata Mannerheim.

### Claim 5 (depends on 1)

A ribonucleic acid (RNA) molecule transcribed from the polynucleotide of claim 1.

### Claim 6 (depends on 1)

A double-stranded ribonucleic acid molecule produced from the expression of the polynucleotide of claim 1.

### Claim 7 (depends on 6)

The double-stranded ribonucleic acid molecule of claim 6, wherein contacting the polynucleotide sequence with coleopteran pest inhibits the expression of an endogenous nucleotide sequence specifically complementary to the polynucleotide.

### Claim 8 (depends on 7)

The double-stranded ribonucleic acid molecule of claim 7, wherein contacting said ribonucleotide molecule with a coleopteran pest kills or inhibits the growth, and/or feeding of the pest.

### Claim 9 (depends on 6)

The double stranded RNA of claim 6, comprising a first, a second and a third RNA segment, wherein the first RNA segment comprises the polynucleotide, wherein the third RNA segment is linked to the first RNA segment by the second polynucleotide sequence, and wherein the third RNA segment is substantially the reverse complement of the first RNA segment, such that the first and the third RNA segments hybridize when transcribed into a ribonucleic acid to form the double-stranded RNA.

### Claim 10 (depends on 5)

The RNA of claim 5, selected from the group consisting of a double-stranded ribonucleic acid molecule and a single-stranded ribonucleic acid molecule of between about 15 and about 30 nucleotides in length.

### Claim 11 (depends on 1)

A plant transformation vector comprising the polynucleotide of claim 1, wherein the heterologous promoter is functional in a plant cell.

### Claim 12 (depends on 1)

A cell transformed with the polynucleotide of claim 1.

### Claim 13 (depends on 12)

The cell of claim 12, wherein the cell is a prokaryotic cell.

### Claim 14 (depends on 12)

The cell of claim 12, wherein the cell is a eukaryotic cell.

### Claim 15 (depends on 14)

The cell of claim 14, wherein the cell is a plant cell.

### Claim 16 (depends on 1)

A plant transformed with the polynucleotide of claim 1.

### Claim 17 (depends on 16)

A seed of the plant of claim 16, wherein the seed comprises the polynucleotide.

### Claim 18 (depends on 16)

A commodity product produced from the plant of claim 16, wherein the commodity product comprises a detectable amount of the polynucleotide.

### Claim 19 (depends on 16)

The plant of claim 16, wherein the at least one polynucleotide is expressed in the plant as a double-stranded ribonucleic acid molecule.

### Claim 20 (depends on 15)

The cell of claim 15, wherein the cell is a maize cell.

### Claim 21 (depends on 16)

The plant of claim 16, wherein the plant is maize.

### Claim 22 (depends on 16)

The plant of claim 16, wherein the at least one polynucleotide is expressed in the plant as a ribonucleic acid molecule, and the ribonucleic acid molecule inhibits the expression of an endogenous polynucleotide that is specifically complementary to the at least one polynucleotide when a coleopteran pest ingests a part of the plant.

### Claim 23 (depends on 1)

The polynucleotide of claim 1, further comprising at least one additional polynucleotide that encodes an RNA molecule that inhibits the expression of an endogenous pest gene.

### Claim 24 (depends on 23)

A plant transformation vector comprising the polynucleotide of claim 23, wherein the additional polynucleotide(s) are each operably linked to a heterologous promoter functional in a plant cell.

### Claim 25 (depends on 1)

A method for controlling a coleopteran pest population, the method comprising:
providing in a host plant of a coleopteran pest a transformed plant cell comprising the polynucleotide of claim 1, wherein the polynucleotide is expressed to produce a ribonucleic acid molecule that functions upon contact with a coleopteran pest belonging to the population to inhibit the expression of a target sequence within the r coleopteran pest and results in decreased growth and/or survival of the coleopteran pest or pest population, relative to the same pest species on a plant of the same host plant species that does not comprise the polynucleotide.

### Claim 26 (depends on 25)

The method according to claim 25, wherein the ribonucleic acid molecule is a double-stranded ribonucleic acid molecule.

### Claim 27 (depends on 25)

The method according to claim 25, wherein the coleopteran pest population is reduced relative to a population of the same pest species infesting a host plant of the same host plant species lacking the transformed plant cell.

### Claim 28 (depends on 25)

The method according to claim 25, wherein the ribonucleic acid molecule is a double-stranded ribonucleic acid molecule.

### Claim 29 (depends on 26)

The method according to claim 26, wherein the coleopteran pest population is reduced relative to a coleopteran pest population infesting a host plant of the same species lacking the transformed plant cell.

### Claim 30 (depends on 1)

A method for improving the yield of a corn crop, the method comprising:
introducing the nucleic acid of claim 1 into a corn plant to produce a transgenic corn plant; and
cultivating the corn plant to allow the expression of the at least one polynucleotide; wherein expression of the at least one polynucleotide inhibits the development or growth of a coleopteran pest and loss of yield due to infection by the coleopteran pest.

### Claim 31 (depends on 30)

The method according to claim 30, wherein expression of the at least one polynucleotide produces an RNA molecule that suppresses at least a first target gene in a coleopteran pest that has contacted a portion of the corn plant.

### Claim 32 (depends on 1)

A method for producing a transgenic plant cell, the method comprising:
transforming a plant cell with a vector comprising the nucleic acid of claim 1;
culturing the transformed plant cell under conditions sufficient to allow for development of a plant cell culture comprising a plurality of transformed plant cells;
selecting for transformed plant cells that have integrated the at least one polynucleotide into their genomes;
screening the transformed plant cells for expression of a ribonucleic acid (RNA) molecule encoded by the at least one polynucleotide; and
selecting a plant cell that expresses the RNA.

### Claim 33 (depends on 32)

The method according to claim 32, wherein the RNA molecule is a double-stranded RNA molecule.

### Claim 34 (depends on 32)

A method for producing a coleopteran pest-resistant transgenic plant, the method comprising:
providing the transgenic plant cell produced by the method of claim 32; and
regenerating a transgenic plant from the transgenic plant cell, wherein expression of the ribonucleic acid molecule encoded by the at least one polynucleotide is sufficient to modulate the expression of a target gene in a coleopteran pest that contacts the transformed plant

### Claim 35

A method for producing a transgenic plant cell, the method comprising:
transforming a plant cell with a vector comprising a means for providing coleopteran pest resistance to a plant;
culturing the transformed plant cell under conditions sufficient to allow for development of a plant cell culture comprising a plurality of transformed plant cells;
selecting for transformed plant cells that have integrated the means for providing coleopteran pest resistance to a plant into their genomes;
screening the transformed plant cells for expression of a means for inhibiting expression of an essential gene in a coleopteran pest; and
selecting a plant cell that expresses the means for inhibiting expression of an essential gene in a coleopteran pest.

### Claim 36 (depends on 44)

A method for producing a coleopteran pest-resistant transgenic plant, the method comprising:
providing the transgenic plant cell produced by the method of claim 44; and
regenerating a transgenic plant from the transgenic plant cell, wherein expression of the means for inhibiting expression of an essential gene in a coleopteran pest is sufficient to modulate the expression of a target gene in a coleopteran pest that contacts the transformed plant.

### Claim 37 (depends on 1)

The nucleic acid of claim 1, further comprising a polynucleotide encoding a polypeptide from Bacillus thuringiensis.

### Claim 38 (depends on 37)

The nucleic acid of claim 37, wherein the polypeptide from B. thuringiensis is selected from a group comprising Cry1, Cry3, Cry6, Cry7, Cry8, Cry9, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, Cyt1, and Cyt2.

### Claim 39 (depends on 15)

The cell of claim 15, wherein the cell comprises a polynucleotide encoding a polypeptide from Bacillus thuringiensis.

### Claim 40 (depends on 39)

The cell of claim 39, wherein the polypeptide from B. thuringiensis is selected from a group comprising Cry1, Cry3, Cry6, Cry7, Cry8, Cry9, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, Cyt1, and Cyt2.

### Claim 41 (depends on 16)

The plant of claim 16, wherein the plant comprises a polynucleotide encoding a polypeptide from Bacillus thuringiensis.

### Claim 42 (depends on 41)

The plant of claim 41, wherein the polypeptide from B. thuringiensis is selected from a group comprising Cry1, Cry3, Cry6, Cry7, Cry8, Cry9, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, Cyt1, and Cyt2.

### Claim 43 (depends on 32)

The method according to claim 32, wherein the transformed plant cell comprises a nucleotide sequence encoding a polypeptide from Bacillus thuringiensis

### Claim 44 (depends on 41)

The method according to claim 41, wherein the polypeptide from B. thuringiensis is selected from a group comprising Cry1, Cry3, Cry6, Cry7, Cry8, Cry9, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, Cyt1, and Cyt2.

### Claim 45

A method for improving the yield of a plant crop, the method comprising:
introducing a nucleic acid of into a corn plant to produce a transgenic plant, wherein the nucleic acid comprises more than one of
a polynucleotide encoding at least one siRNA targeting a gene selected from the group consisting of: chitin synthase, outer membrane translocase, double parked, discs overgrown, ctf4, rpl9, serpin protease inhibitor I4, myosin 3 LC, megator, g-protein beta subunit, flap wing, female sterile 2 ketel, enhancer of polycomb, dead box 73D, cg7000, heat shock protein 70-331, heat shock protein 70-12300, rnr1, elav, pten, and cdc8;
a polynucleotide encoding an insecticidal polypeptide from Bacillus thuringiensis, and
cultivating the plant to allow the expression of the at least one polynucleotide; wherein expression of the at least one polynucleotide inhibits coleopteran pest development or growth and loss of yield due to coleopteran pest infection.

### Claim 46 (depends on 43)

The method according to claim 43, wherein the plant is maize, soybean or cotton.

### Claim 47 (depends on 6)

The double stranded RNA of claim 6, comprising a first, a second and a third RNA segment, wherein the first RNA segment comprises the polynucleotide, wherein the third RNA segment is linked to the first RNA segment by the second polynucleotide sequence, and wherein the third RNA segment is substantially the reverse complement of the first RNA segment, such that the first and the third RNA segments hybridize when transcribed into a ribonucleic acid to form the double-stranded RNA.