# US 2021/0277380 A1 · Aggregate of cell carrier particles and method for preparing same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0277380 A1 - Document ID: US20210277380A1 - Status: Published application - Kind: A1 - Application number: 17/303,130 - Filed: 2021-05-20 - Published: 2021-09-09 - Earliest claimed: 2019-01-28 - Assignees: Beijing CytoNiche Biotechnology Co., Ltd. - Applicants: Beijing CytoNiche Biotechnology Co., Ltd. - Inventors: Wei LIU; Xiaojun YAN - Web page: https://patentgrep.com/patent/US20210277380A1 ## CPC - C12N 5/0075: {using microcarriers} - C12N 11/02: Enzymes or microbial cells immobilised on or in an organic carrier - B29B 11/06: by moulding the material - B29C 41/003: {characterised by the choice of material} - B29C 41/38: Moulds, cores or other substrates - C12M 25/16: {Particles; Beads; Granular material; Encapsulation (chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00)} - B29K 2089/00: Use of proteins, e.g. casein, gelatine or derivatives thereof, as moulding material - B29K 2833/12: Polymers of methacrylic acid esters, e.g. PMMA, i.e. polymethylmethacrylate - C12N 2533/52: Fibronectin; Laminin - C12N 2533/54: Collagen; Gelatin - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - B29B: PREPARATION OR PRETREATMENT OF THE MATERIAL TO BE SHAPED; MAKING GRANULES OR PREFORMS; RECOVERY OF PLASTICS OR OTHER CONSTITUENTS OF WASTE MATERIAL CONTAINING PLASTICS - B29C: SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING (making preforms B29B11/00; making laminated products by combining previously unconnected layers which become one product whose layers will remain together B32B37/00 - B32B41/00) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - B29K: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES B29B, B29C OR B29D, RELATING TO MOULDING MATERIALS OR TO MATERIALS FOR {MOULDS, } REINFORCEMENTS, FILLERS OR PREFORMED PARTS, e.g. INSERTS ## Abstract The present disclosure discloses an aggregate of cell carrier particles and a method for preparing same. The aggregate of cell carrier particles is formed by aggregating cell carrier particles and has a particular shape including the shape of a tablet and the shape of a block. The method for preparing the aggregate of cell carrier particles is a punch-forming process, a mold-forming process, a lyophilization process or a dehydrating-evaporating process. ## Claims ### Claim 1 An aggregate of cell carrier particles, wherein the aggregate of cell carrier particles is formed by aggregating cell carrier particles. ### Claim 2 (depends on 1) The aggregate of cell carrier particles according to claim 1, further comprising the aggregate of cell carrier particles having a particular shape. ### Claim 3 (depends on 2) The aggregate of cell carrier particles according to claim 2, wherein the particular shape further comprises the shape of a tablet or a block; and the aggregate of cell carrier particles has an excellent rehydration property and a good dispersibility upon rehydration. ### Claim 4 A method for preparing an aggregate of cell carrier particles, comprising: aggregating and shaping cell carrier particles under an external force to obtain the aggregate of cell carrier particles. ### Claim 5 (depends on 4) The method according to claim 4, further comprising the cell carrier particles are aggregated and shaped by a punch-forming process. ### Claim 6 (depends on 5) The method according to claim 5, further comprising performing the punch-forming process under the following conditions: the punching mold is selected from a beveled flat punching mold, a shallow arc punching mold, a deep arc punching mold, or a full flat punching mold; the punch-forming machine has an upper punch with an adjusting range between 0 and 50 mm, and a lower punch with an adjusting range between 0 and 50 mm; and the pressure ranges from 0 to 200 KN. ### Claim 7 (depends on 4) The method according to claim 4, further comprising the cell carrier particles are aggregated and shaped by a mold-forming process. ### Claim 8 (depends on 7) The method according to claim 7, wherein the mold-forming process comprises the following steps of: mixing the cell carrier particles with water or a volatile organic solvent and filling the resultant mixture into a mold; and then placing the mold in an oven and drying to obtain the aggregate of cell carrier particles. ### Claim 9 (depends on 8) The method according to claim 8, further comprising the water or the organic solvent is added in an amount of 5 to 100 times of the mass of the cell carrier particles; and the temperature of the oven is 30 to 200° C., and the drying is performed for a period of 12 to 96 hours. ### Claim 10 (depends on 4) The method according to claim 4, wherein the cell carrier particles are aggregated and shaped by a lyophilization process; wherein the lyophilization process comprises the following steps of: mixing the cell carrier particles with water or a volatile organic solvent and shaping the resultant mixture into a particular shape, followed by freezing the shaped mixture to obtain a frozen mixture; and lyophilizing the frozen mixture to obtain the aggregate of cell carrier particles. ### Claim 11 (depends on 10) The method according to claim 10, wherein the water or the organic solvent is added in an amount of 5 to 100 times of the mass of the cell carrier particles; the shaped mixture is frozen in a freezing container at a low temperature ranging from −196° C. to 0° C. to obtain the frozen mixture; and the frozen mixture is lyophilized in a lyophilizer for a period of 2 to 96 hours to obtain the aggregate of cell carrier particles. ### Claim 12 (depends on 4) The method according to claim 4, wherein the cell carrier particles are aggregated and shaped by a dehydrating-evaporating process comprising the following steps of: mixing the cell carrier particles with water or a volatile organic solvent and shaping the mixture into a particular shape; and then dehydrating and evaporating the shaped mixture to obtain the aggregate of cell carrier particles. ### Claim 13 (depends on 12) The method according to claim 12, characterized in that the water or the organic solvent is added in an amount of 5 to 100 times of the mass of the cell carrier particles. ### Claim 14 (depends on 13) The method according to claim 13, wherein the cell carrier particles are aggregated and shaped by natural dehydration at room temperature. ### Claim 15 (depends on 13) The method according to claim 13, wherein the cell carrier particles are aggregated and shaped by drying in an oven.