# US 2021/0277375 A1 · Method for manufacturing a stable aqueous solution of beta-amylase, aqueous solution obtained and uses thereof

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- Number: US 2021/0277375 A1
- Document ID: US20210277375A1
- Status: Published application
- Kind: A1
- Application number: 17/238,662
- Filed: 2021-04-23
- Published: 2021-09-09
- Earliest claimed: 2014-06-16
- Assignees: Roquette Freres
- Applicants: Roquette Freres
- Inventors: Vincent COURBOIS; Aline LECOCQ; Pierrick DUFLOT
- Web page: https://patentgrep.com/patent/US20210277375A1

## CPC

- C12N 9/2425: {Beta-amylase (3.2.1.2)}
- A23L 27/30: Artificial sweetening agents
- A23L 29/35: {Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches}
- C12N 9/96: Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
- C12Y 302/01002: Beta-amylase (3.2.1.2)
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12Y: ENZYMES
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The present invention relates to a method for stabilising an aqueous solution of β-amylase, in particular by the use of glycerol, potassium sorbate and sodium carbonate. Said cocktail of additives is particularly effective at maintaining the enzymatic activity of β-amylase over time. Another aim of the present invention consists of using said cocktail for the specific function of maintaining the enzymatic activity of the β-amylase. Another aim of the present invention is to provide an aqueous solution of β-amylase containing the aforementioned cocktail. A final aim of the present invention consists of using said β-amylase aqueous solution in bread-making, in malting, as a food additive, as a digestive agent, for sweetener production, in pharmacy and, finally, for maltose and maltose-enriched syrup production.

## Claims

### Claim at position 1

1-10. (canceled)

### Claim 11

A method for the production of maltose or maltose enriched syrups, comprising adding the aqueous solution of β-amylase to maltose or a maltose enriched syrups, said aqueous solution of β-amylase, said solution containing:
a) potassium sorbate;
b) glycerol; and
c) sodium carbonate.

### Claim 12 (depends on 11)

The method as claimed in claim 11, characterized in that said solution contains:
a) from 0.05 to 1% potassium sorbate;
b) from 30 to 60% glycerol; and
c) from 0.05 to 1% sodium carbonate
these % being expressed as % by dry weight of each constituent relative to the total weight of said aqueous solution.

### Claim 13 (depends on 11)

The method as claimed in claim 11, characterized in that said solution contains:
a) from 0.05 to 0.5% potassium sorbate;
b) from 30 to 50% glycerol;
c) from 0.05 to 0.5% sodium carbonate;
these % being expressed as % by dry weight of each constituent relative to the total weight of said aqueous solution.

### Claim 14 (depends on 11)

The method as claimed in claim 11, characterized in that said solution contains:
a) from 0.2 to 1% of potassium sorbate;
b) from 40 to 60% of glycerol;
c) from 0.2 to 1% of sodium carbonate;
these % being expressed as % by dry weight of each constituent relative to the total weight of said aqueous solution.

### Claim 15 (depends on 11)

The method as claimed in claim 11, characterized in that said solution contains:
a) from 0.1 to 0.3% of potassium sorbate;
b) from 35 to 45% of glycerol;
c) from 0.1 to 0.3% of sodium carbonate;
these % being expressed as % by dry weight of each constituent relative to the total weight of said aqueous solution.

### Claim 16 (depends on 11)

The method as claimed in claim 11, characterized in that said solution contains:
a) approximately 0.2% potassium sorbate;
b) approximately 40% glycerol;
c) approximately 0.2% sodium carbonate;
these % being expressed as % by dry weight of each constituent relative to the total weight of said aqueous solution.

### Claim 17 (depends on 11)

The method as claimed in claim 11, characterized in that said solution has a content by dry weight of β-amylase of between 5 and 20% of the total weight thereof.

### Claim 18 (depends on 11)

The method as claimed in claim 11, characterized in that said solution has a content by dry weight of β-amylase of between 10 and 20% of the total weight thereof.

### Claim 19 (depends on 11)

The method as claimed in claim 11, characterized in that said solution has a content by dry weight of β-amylase of approximately 15% of the total weight thereof.

### Claim 20 (depends on 11)

The method as claimed in claim 11, characterized in that the β-amylase is obtained from a soluble fraction of starch plants.

### Claim 21 (depends on 11)

The method as claimed in claim 11, characterized in that said aqueous solution of β-amylase is obtained by the steps consisting in:
providing a soluble fraction of starch plants;
carrying out a microfiltration step on said soluble fraction, in order to obtain a microfiltration permeate;
carrying out an ultrafiltration step on the microfiltration permeate, in order to obtain an ultrafiltration retentate.